Publication: Modulation of bovine herpesvirus 1 infection by virally encoded microRNAs
Issued Date
2018-09-15
Resource Type
ISSN
18727492
01681702
01681702
Other identifier(s)
2-s2.0-85052968257
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Virus Research. Vol.257, (2018), 1-6
Suggested Citation
Sitthichai Kanokudom, Timothy J. Mahony, Duncan R. Smith, Wanchai Assavalapsakul Modulation of bovine herpesvirus 1 infection by virally encoded microRNAs. Virus Research. Vol.257, (2018), 1-6. doi:10.1016/j.virusres.2018.08.022 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45052
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Modulation of bovine herpesvirus 1 infection by virally encoded microRNAs
Other Contributor(s)
Abstract
© 2018 Elsevier B.V. Bovine herpesvirus 1 (BoHV-1), is a member of the subfamily Alphaherpesvirinae in the order Herpesviridae and is a ubiquitous pathogen of cattle responsible for significant economic loss worldwide. The BoHV-1 genome encodes at least 10 BoHV-1 microRNA (miRNA) genes, whose functions remain poorly understood. This study sought to understand the role of three BoHV-1 miRNA genes, Bhv1-miR-B6, Bhv1-miR-B8 and Bhv1-miR-B9, which are located proximal to the BoHV-1 origins of replication (OriS). Therefore, plasmids expressing the precursor miRNA hairpins for the Bhv1-miR-B6, Bhv1-miR-B8, and Bhv1-miR-B9 genes were constructed and transfected into Madin-Darby bovine kidney cells prior to BoHV-1 infection. Interestingly, transient expression of either Bhv1-miR-B8 or Bhv1-miR-B9 in Madin-Darby bovine kidney cells prior to infection resulted in partial suppression of BoHV-1 replication, quantified through estimating levels of glycoprotein C mRNA and protein levels. Putative interactions between the mature miRNA bhv1-miR-B8-3p and bhv1-miR-B9 and BoHV-1 transcripts were identified providing plausible pathways for these molecules to affect virus replication. Therefore, these two miRNAs are implicated in the post-transcriptional regulation of BoHV-1 transcripts important for virus replication and could be used to limit BoHV-1 replication.