Publication: Validation of osmotic fragility test and dichlorophenol indophenol precipitation test for screening of thalassemia and Hb E
Issued Date
2005-11-01
Resource Type
ISSN
01251562
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2-s2.0-33645021326
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.6 (2005), 1538-1542
Suggested Citation
Siripakorn Sangkitporn, Somchai Sangkitporn, Areerat Sangnoi, Ornchira Supangwiput, Voravarn S. Tanphaichitr Validation of osmotic fragility test and dichlorophenol indophenol precipitation test for screening of thalassemia and Hb E. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.6 (2005), 1538-1542. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16766
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Title
Validation of osmotic fragility test and dichlorophenol indophenol precipitation test for screening of thalassemia and Hb E
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Abstract
The strategy for screening of thalassemia and Hb E by a combination of osmotic fragility (OF) test and dichlorophenol indophenol precipitation (DCIP) test was validated with 436 unrelated Thai subjects. Hemoglobin (Hb) typing, Hb A2 quantitation, PCR and DNA sequence analysis were used as confirmatory methods for diagnosis of thalassemia and hemoglobinopathy. The sensitivity and specificity of this strategy was 100% and 79.7%, respectively. The results assessed by two medical scientists were exactly the same with 93.3% accuracy in comparison with the confirmatory methods. A combination of OF and DCIP has been shown to be a reliable, rapid, simple and sensitive strategy for screening thalassemia and Hb E in the Thai population.
