Publication: Effects of vitrification cryoprotectant treatment and cooling method on the viability and development of buffalo oocytes after intracytoplasmic sperm injection
Issued Date
2012-10-01
Resource Type
ISSN
10902392
00112240
00112240
Other identifier(s)
2-s2.0-84864316411
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Mahidol University
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SCOPUS
Bibliographic Citation
Cryobiology. Vol.65, No.2 (2012), 151-156
Suggested Citation
Yuan Yuan Liang, Kanokwan Srirattana, Tatsanee Phermthai, Tamas Somfai, Takashi Nagai, Rangsun Parnpai Effects of vitrification cryoprotectant treatment and cooling method on the viability and development of buffalo oocytes after intracytoplasmic sperm injection. Cryobiology. Vol.65, No.2 (2012), 151-156. doi:10.1016/j.cryobiol.2012.04.006 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13392
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Title
Effects of vitrification cryoprotectant treatment and cooling method on the viability and development of buffalo oocytes after intracytoplasmic sperm injection
Abstract
In vitro matured (IVM) buffalo oocytes at the metaphase of the second meiotic division (MII) were vitrified in 20% Me 2 SO: 20% EG (v/v) and 0.5M sucrose (VA), or 35% EG (v/v), 50mg/mL polyvinylpyrrolidone (PVP), and 0.4M trehalose (VB), either on cryotops or as 2μL microdrops. The viability was assessed after warming by fluorescein diacetate (FDA) staining and all surviving oocytes were subjected to ICSI and ethanol activation. All vitrified groups had similar recovery rates but both VA groups had significantly higher survival and pronuclear formation rates than either of the VB groups. Non treated control oocytes and non cryopreserved oocytes exposed to FDA had significantly higher survival, 2nd polar body extrusion, PN and blastocyst formation rates than any of the four vitrified groups (P < 0.05). In conclusion The cryotop and microdrop methods are equally effective for buffalo oocyte vitrification, and although vitrification in VA solution yielded higher rates of survival and formation of 2 pronuclei than VB, the rate of blastocyst formation was comparable for both solutions. A detailed analysis of oocytes that extruded the second polar body after ICSI and activation revealed that only a minority (7-20% of the vitrified and 46-48% of the control oocytes) also had two pronuclei, indicating that normal activation is compromised by vitrification. © 2012 Elsevier Inc.