Publication: Investigation of the cry4B-prohibitin interaction in Aedes aegypti cells
Issued Date
2012-10-01
Resource Type
ISSN
14320991
03438651
03438651
Other identifier(s)
2-s2.0-84865972477
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Current Microbiology. Vol.65, No.4 (2012), 446-454
Suggested Citation
Atichat Kuadkitkan, Duncan R. Smith, Colin Berry Investigation of the cry4B-prohibitin interaction in Aedes aegypti cells. Current Microbiology. Vol.65, No.4 (2012), 446-454. doi:10.1007/s00284-012-0178-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14254
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Investigation of the cry4B-prohibitin interaction in Aedes aegypti cells
Author(s)
Other Contributor(s)
Abstract
Bacillus thuringiensis (Bt) produces insecticidal toxins active against insects. Cry4B, one of the major insecticidal toxins produced by Bt subsp. israelensis, is highly toxic to mosquitoes in the genus Aedes: the major vectors of dengue, yellow fever, and chikungunya. Previous work has shown that Cry4B binds to several mid-gut membrane proteins in Aedes aegypti larvae including prohibitin, a protein recently identified as a receptor that also mediates entry of dengue virus into Aedes cells. This study confirms the interaction between Cry4B and prohibitin by co-immunoprecipitation analysis and demonstrates colocalization of prohibitin and Cry4B by confocal microscopy. While activated Cry4B toxin showed high larvicidal activity, it was not cytotoxic to two Aedes cell lines, allowing determination of its effect on dengue virus infectivity in the absence of Cry4B-induced cell lysis. Preexposure of Aedes cells to Cry4B resulted in a significant reduction in the number of infected cells compared to untreated cells. © 2012 Springer Science+Business Media, LLC.