Dengue virus - Induced hemorrhage in a nonhuman primate model

dc.contributor.authorNattawat Onlamoonen_US
dc.contributor.authorSansanee Noisakranen_US
dc.contributor.authorHui Mien Hsiaoen_US
dc.contributor.authorAlexander Duncanen_US
dc.contributor.authorFrancois Villingeren_US
dc.contributor.authorAftab A. Ansarien_US
dc.contributor.authorGuey Chuen Perngen_US
dc.contributor.otherEmory University School of Medicineen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThailand National Center for Genetic Engineering and Biotechnologyen_US
dc.contributor.otherEmory Universityen_US
dc.description.abstractLack of a dengue hemorrhagic animal model recapitulating human dengue virus infection has been a significant impediment in advancing our understanding of the early events involved in the pathogenesis of dengue disease. In efforts to address this issue, a group of rhesus macaques were intravenously infected with dengue virus serotype 2 (strain 16 681) at 1 × 107 PFU/animal. A classic dengue hemorrhage developed 3 to 5 days after infection in 6 of 6 animals. Blood chemistry appeared to be normal with exception of creatine phosphokinase, which peaked at 7 days after infection. A modest thrombocytopenia and noticeable neutropenia concomitant with slight decrease of hemoglobin and hematocrit were registered. In addition, the concentration of D-dimer was elevated significantly. Viremia peaked at 3 to 5 days after infection followed by an inverse relationship between T and B lymphocytes and a bimodal pattern for platelet-monocytes and platelet-neutrophil aggregates. Dengue virus containing platelets engulfed by monocytes was noted at 8 or 9 days after infection. Thus, rhesus macaques inoculated intravenously with a high dose of dengue virus produced dengue hemorrhage, which may provide a unique platform to define the early events in dengue virus infection and help identify which blood components contribute to the pathogenesis of dengue disease. © 2010 by The American Society of Hematology.en_US
dc.identifier.citationBlood. Vol.115, No.9 (2010), 1823-1834en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleDengue virus - Induced hemorrhage in a nonhuman primate modelen_US