Publication: Diagnosis of gnathostomiasis by skin testing using partially purified specific antigen and total IgE levels
Issued Date
2014-02-01
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ISSN
18783503
00359203
00359203
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2-s2.0-84892963712
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Mahidol University
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SCOPUS
Bibliographic Citation
Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.108, No.2 (2014), 71-76
Suggested Citation
Wirach Maek-a-nantawat, Valai Bussaratid, Benjaluck Phonrat, Wallop Pakdee, Supaporn Nuamtanong, Paron Dekumyoy Diagnosis of gnathostomiasis by skin testing using partially purified specific antigen and total IgE levels. Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.108, No.2 (2014), 71-76. doi:10.1093/trstmh/trt118 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/33992
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Title
Diagnosis of gnathostomiasis by skin testing using partially purified specific antigen and total IgE levels
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Abstract
Background: A finding of antibodies to Gnathostoma spinigerum 24-kDa antigen by immunoblot analysis is currently used to confirm a diagnosis of gnathostomiasis. A simple skin test for the diagnosis of gnathostomiasis was developed, and the results were evaluated and compared with the standard Western blot (WB) test. Methods: This cross-sectional study was conducted at the Hospital for Tropical Diseases, Bangkok, Thailand, in 2008-2011. All eligible patients were tested with partially purified proteins of mAb-detected fractions pooled and sterilized by 0.2 mm diameter syringe filter, with a phenol saline solution of 1:10 w/v. Results: A total of 69 cases, 39 gnathostomiasis cases and 30 controls, were enrolled into the study; the median age (IQR) was 40 (30.5-52.5) years. The most common presenting symptom was edema (56/69, 81%). Gnathostomiasis cases having strong cutaneous reactions to the intradermal test (81%) were also positive by immunoblot. A significant correlation between skin and immunoblot tests was detected (p<0.001). The difference in total IgE levels between cases and controls was not statistically significant (p=0.51). Logistic regression models showed that positive WB and skin-test results were significantly associated with gnathostomiasis (p=0.001 and p=0.007, respectively). Conclusion: Gnathostoma skin testing, using prepared fractionated antigen solution of Gnathostoma spinigerum, yields good reactivity and significantly correlates with the results of immunoblot testing. © The Author 2013. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved.