Publication: An antibiotic selection marker for schistosome transgenesis
Issued Date
2012-01-01
Resource Type
ISSN
18790135
00207519
00207519
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2-s2.0-84855200901
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal for Parasitology. Vol.42, No.1 (2012), 123-130
Suggested Citation
Gabriel Rinaldi, Sutas Suttiprapa, José F. Tort, Anne E. Folley, Danielle E. Skinner, Paul J. Brindley An antibiotic selection marker for schistosome transgenesis. International Journal for Parasitology. Vol.42, No.1 (2012), 123-130. doi:10.1016/j.ijpara.2011.11.005 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/14364
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Title
An antibiotic selection marker for schistosome transgenesis
Abstract
Drug selection is widely used in transgene studies of microbial pathogens, mammalian cell and plant cell lines. Drug selection of transgenic schistosomes would be desirable to provide a means to enrich for populations of transgenic worms. We adapted murine leukaemia retrovirus vectors - widely used in human gene therapy research - to transduce schistosomes, leading to integration of transgenes into the genome of the blood fluke. A dose-response kill curve and lethal G418 (geneticin) concentrations were established: 125-1,000 μg/ml G418 were progressively more toxic for schistosomules of Schistosoma mansoni with toxicity increasing with antibiotic concentration and with duration of exposure. By day 6 of exposure to ≥500 μg/ml, significantly fewer worms survived compared with non-exposed controls and by day 8, significantly fewer worms survived than controls at ≥250 μg/ml G418. When schistosomules were transduced with murine leukaemia retrovirus encoding the neomycin resistance (neoR) transgene and cultured in media containing G418, the neoR transgene rescued transgenic schistosomules from the antibiotic; by day 4 in 1,000 μg/ml and by day 8 in 500 μg/ml G418, significantly more transgenic worms survived the toxic effects of the antibiotic. More copies of neoR were detected per nanogram of genomic DNA from populations of transgenic schistosomes cultured in G418 than from transgenic schistosomes cultured without G418. This trend was G418 dose-dependent, demonstrating enrichment of transgenic worms from among the schistosomules exposed to virions. Furthermore, higher expression of neoR was detected in transgenic schistosomes cultured in the presence of G418 than in transgenic worms cultured without antibiotic. The availability of antibiotic selection can be expected to enhance progress with functional genomics research on the helminth parasites responsible for major neglected tropical diseases. © 2011 Australian Society for Parasitology Inc.