Publication: Oncoproteomic and gene expression analyses identify prognostic biomarkers for second primary malignancy in patients with head and neck squamous cell carcinoma
Issued Date
2019-07-01
Resource Type
ISSN
15300285
08933952
08933952
Other identifier(s)
2-s2.0-85061275882
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Modern Pathology. Vol.32, No.7 (2019), 943-956
Suggested Citation
Sacarin Bunbanjerdsuk, Nutchavadee Vorasan, Thammakorn Saethang, Tanjitti Pongrujikorn, Duangjai Pangpunyakulchai, Narongsak Mongkonsiri, Lalida Arsa, Nintita Thokanit, Warut Pongsapich, Tauangtham Anekpuritanang, Nuttapong Ngamphaiboon, Artit Jinawath, Somkiat Sunpaweravong, Trairak Pisitkun, Bhoom Suktitipat, Natini Jinawath Oncoproteomic and gene expression analyses identify prognostic biomarkers for second primary malignancy in patients with head and neck squamous cell carcinoma. Modern Pathology. Vol.32, No.7 (2019), 943-956. doi:10.1038/s41379-019-0211-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/51578
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Oncoproteomic and gene expression analyses identify prognostic biomarkers for second primary malignancy in patients with head and neck squamous cell carcinoma
Author(s)
Sacarin Bunbanjerdsuk
Nutchavadee Vorasan
Thammakorn Saethang
Tanjitti Pongrujikorn
Duangjai Pangpunyakulchai
Narongsak Mongkonsiri
Lalida Arsa
Nintita Thokanit
Warut Pongsapich
Tauangtham Anekpuritanang
Nuttapong Ngamphaiboon
Artit Jinawath
Somkiat Sunpaweravong
Trairak Pisitkun
Bhoom Suktitipat
Natini Jinawath
Nutchavadee Vorasan
Thammakorn Saethang
Tanjitti Pongrujikorn
Duangjai Pangpunyakulchai
Narongsak Mongkonsiri
Lalida Arsa
Nintita Thokanit
Warut Pongsapich
Tauangtham Anekpuritanang
Nuttapong Ngamphaiboon
Artit Jinawath
Somkiat Sunpaweravong
Trairak Pisitkun
Bhoom Suktitipat
Natini Jinawath
Abstract
© 2019, United States & Canadian Academy of Pathology. Patients with head and neck squamous cell carcinoma are at increased risk of developing a second primary malignancy, which is associated with poor prognosis and early death. To help improve clinical outcome, we aimed to identify biomarkers for second primary malignancy risk prediction using the routinely obtained formalin-fixed paraffin-embedded tissues of the index head and neck cancer. Liquid chromatography-tandem mass spectrometry was initially performed for candidate biomarker discovery in 16 pairs of primary cancer tissues and their matched normal mucosal epithelia from head and neck squamous cell carcinoma patients with or without second primary malignancy. The 32 candidate proteins differentially expressed between head and neck cancers with and without second primary malignancy were identified. Among these, 30 selected candidates and seven more from literature review were further studied using NanoString nCounter gene expression assay in an independent cohort of 49 head and neck cancer patients. Focusing on the p16-negative cases, we showed that a multivariate logistic regression model comprising the expression levels of ITPR3, KMT2D, EMILIN1, and the patient’s age can accurately predict second primary malignancy occurrence with 88% sensitivity and 75% specificity. Furthermore, using Cox proportional hazards regression analysis and survival analysis, high expression levels of ITPR3 and DSG3 were found to be significantly associated with shorter time to second primary malignancy development (log-rank test P = 0.017). In summary, we identified a set of genes whose expressions may serve as the prognostic biomarkers for second primary malignancy occurrence in head and neck squamous cell carcinomas. In combination with the histopathologic examination of index tumor, these biomarkers can be used to guide the optimum frequency of second primary malignancy surveillance, which may lead to early diagnosis and better survival outcome.