Publication: Evaluation of the panbio leptospira IgM ELISA among outpatients attending primary care in Southeast Asia
Issued Date
2021-05-05
Resource Type
ISSN
14761645
00029637
00029637
Other identifier(s)
2-s2.0-85105544704
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.104, No.5 (2021), 1777-1781
Suggested Citation
Sandhya Dhawan, Thomas Althaus, Yoel Lubell, Duangjai Suwancharoen, Stuart D. Blacksell Evaluation of the panbio leptospira IgM ELISA among outpatients attending primary care in Southeast Asia. American Journal of Tropical Medicine and Hygiene. Vol.104, No.5 (2021), 1777-1781. doi:10.4269/ajtmh.20-0818 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/77289
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Evaluation of the panbio leptospira IgM ELISA among outpatients attending primary care in Southeast Asia
Abstract
Despite estimates suggesting Leptospira spp. being endemic in Southeast Asia, evidence remains limited. Diagnostic accuracy evaluations based on Leptospira ELISA mainly rely on hospitalized and severe patients; therefore, studies measuring the pathogen burden may be inaccurate in the community. Weevaluated the Panbio Leptospira ELISA IgM among 656 febrile outpatients attending primary care in Chiangrai, Thailand, and Hlaing Tha Yar, Yangon, Myanmar. ELISA demonstrated limited diagnostic accuracy for the detection of acute leptospiral infection using the manufacturer recommended cutoff, with a sensitivity of 71.4% and specificity of 36.4%, and an area under the receiver operator characteristic curve value of 0.65 (95% CI: 0.41-0.89), compared with our reference test, the PCR assay. ELISA also performed poorly as a screening tool for detecting recent exposure to Leptospira spp. compared with the "gold-standard"microscopic agglutination test, with a specificity of 42.7%. We conclude that the utility of the Leptospira IgM ELISA for both serodiagnosis and seroprevalence is limited in our setting.