Publication: A rapid measurement of apoptosis-associated light scatter changes using a hematology analyzer
2
Issued Date
2000-06-15
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ISSN
01964763
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2-s2.0-0034659293
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Mahidol University
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SCOPUS
Bibliographic Citation
Communications in Clinical Cytometry. Vol.42, No.3 (2000), 215-217
Suggested Citation
Thawatchai Lertworasirikul, Ahnond Bunyaratvej A rapid measurement of apoptosis-associated light scatter changes using a hematology analyzer. Communications in Clinical Cytometry. Vol.42, No.3 (2000), 215-217. doi:10.1002/1097-0320(20000615)42:3<215::AID-CYTO8>3.0.CO;2-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/25865
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Title
A rapid measurement of apoptosis-associated light scatter changes using a hematology analyzer
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Abstract
The alternative application of an automated hematology analyzer, H*3 system, has been described for the detection of apoptosis. Apoptosis induction by the topoisomerase I inhibitor, camptothecin (CAM) on several cell lines is followed by typical morphological alterations. On the H*3 cytogram, measurement of CAM-treated cells revealed an increased population of cells with reduced size suggesting cell contraction during apoptosis. The decreased LUC/Lymph ratio also indicated the enhanced degree of apoptosis directly correlated with increasing CAM concentration anti/or incubation period. Quantitative analysis shows a good correlation between the H*3 measurement and flow cytometry measurements of Annexin V-fluorescein isothiocyanate-labeled method. Thus, the H*3 measurement, under an appropriate adjustment, can be used as a rapid monitor for evaluating the degree of apoptotic changes in drug susceptibility testing of homogeneous cell samples. (C) 2000 Wiley-Liss, Inc.