Publication: Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor
2
Issued Date
2019-05-01
Resource Type
ISSN
18734235
09565663
09565663
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2-s2.0-85062811982
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Mahidol University
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SCOPUS
Bibliographic Citation
Biosensors and Bioelectronics. Vol.132, (2019), 271-278
Suggested Citation
Jantana Kampeera, Patiya Pasakon, Chanpen Karuwan, Narong Arunrut, Assawapong Sappat, Sarawut Sirithammajak, Nipaphorn Dechokiattawan, Thitinun Sumranwanich, Parin Chaivisuthangkura, Puey Ounjai, Surang Chankhamhaengdecha, Anurat Wisitsoraat, Adisorn Tuantranont, Wansika Kiatpathomchai Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor. Biosensors and Bioelectronics. Vol.132, (2019), 271-278. doi:10.1016/j.bios.2019.02.060 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/50190
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Title
Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor
Abstract
© 2019 Elsevier B.V. Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes (SPGEs). The LAMP reactions using primers targeting V. parahaemolyticus toxR gene were optimized at an isothermal temperature of 65 °C, providing specific detection of V. parahaemolyticus within 45 min at the detection limit of 0.3 CFU per 25 g of raw seafood. The LAMP amplicons can be effectively detected using unmodified SPGEs, redox active molecules namely Hoechst-33258 and a portable potentiostat. Therefore, the proposed system is particularly suitable as a point-of-care device for on-site detection of foodborne pathogens.