Publication: Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta
Issued Date
2017-11-01
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14665026
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2-s2.0-85034215022
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Systematic and Evolutionary Microbiology. Vol.67, No.11 (2017), 4572-4577
Suggested Citation
Watsapon Wattanasuepsin, Bungonsiri Intra, Akira Také, Yuki Inahashi, Jirayut Euanorasetr, Satoshi Ōmura, Atsuko Matsumoto, Watanalai Panbangred Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta. International Journal of Systematic and Evolutionary Microbiology. Vol.67, No.11 (2017), 4572-4577. doi:10.1099/ijsem.0.002336 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41316
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Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta
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Abstract
© 2017 IUMS. A non-Streptomyces actinomycete, designated as strain S265T, was isolated from rhizosphere collected under an elephant ear plant (Colocasia esculenta) in Bangkok, Thailand. The taxonomic position of this strain was determined by a polyphasic approach. Strain S265T formed single globose spores on long, branching, aerial hyphae. It produced abundant aerial mycelium with green colour. The cell wall contained meso-diaminopimelic acid, and diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylethanolamine and diphosphatidylglycerol were detected predominantly as polar lipids, whereas mycolic acids were not found. The major menaquinone was MK-9(H4), and principal cellular fatty acids were C15: 1 B, iso-C16: 1 H, anteiso-C15: 0 and C15: 0 2-OH. The DNA G+C content was 69 mol%. According to phylogenetic analysis, strain S265T was clustered with Saccharomonospora glauca K62T (98.1%) and Saccharomonospora viridis DSM 43017T (97.1%) despite its 16S rRNA gene sequence showing the highest similarity value to that of Saccharomonospora azurea NA-128T (98.6%). DNA–DNA relatedness values between strain S265T and the closely related strains were in the range of 7–50%, thus strengthening the evidence derived from the polyphasic study that strain S265T represents a novel species within the genus Saccharomonospora, for which the name Saccharomonospora colocasiae sp. nov. is proposed. The type strain is S265T (=TBRC 7235T=NBRC 112945T).