Publication: Green and sustainable biocatalytic production of 3,4,5-trihydroxycinnamic acid from palm oil mill effluent
Issued Date
2017-12-01
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ISSN
13595113
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2-s2.0-85029173905
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Mahidol University
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SCOPUS
Bibliographic Citation
Process Biochemistry. Vol.63, (2017), 122-129
Suggested Citation
Chatchadaporn Pinthong, Pattamaporn Phoopraintra, Rattikan Chantiwas, Thunyarat Pongtharangkul, Pirom Chenprakhon, Pimchai Chaiyen Green and sustainable biocatalytic production of 3,4,5-trihydroxycinnamic acid from palm oil mill effluent. Process Biochemistry. Vol.63, (2017), 122-129. doi:10.1016/j.procbio.2017.08.006 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41654
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Title
Green and sustainable biocatalytic production of 3,4,5-trihydroxycinnamic acid from palm oil mill effluent
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Abstract
© 2017 Elsevier Ltd Palm oil mill effluent (POME) is a major waste product of the palm oil industry. This work describes the development and utilization of an effective protocol to analyze and extract phenolic acids from POME. p-Coumaric acid (CMA) and caffeic acid (CFA) were identified as major constituents. We therefore developed a protocol to synthesize 3,4,5-trihydroxycinnamic acid (3,4,5-THCA), a strong antioxidant with potential use as a medicinal agent, based on the biocatalytic reaction of p-hydroxyphenylacetate hydroxylase (HPAH) with CMA as a substrate. The reaction showed greater efficiency than previous protocols in terms of cost (1/10 reduction), scale (40–50 fold increase) and production time (1/3 reduction). The protocol could be used successfully in the bioconversion of CMA and CFA extracted from POME to generate 3,4,5-THCA. This is the first report to demonstrate the production of 3,4,5-THCA from a renewable and sustainable biomass feedstock using a method that abides by all of the principles of green chemistry, paving the road for future development so that palm oil industries and farmers can receive high economic benefit.