Publication: Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Issued Date
2019-10-01
Resource Type
ISSN
15214141
00142980
00142980
Other identifier(s)
2-s2.0-85073598520
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
European Journal of Immunology. Vol.49, No.10 (2019), 1457-1973
Suggested Citation
Andrea Cossarizza, Hyun Dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, Sabine Adam-Klages, William W. Agace, Nima Aghaeepour, Mübeccel Akdis, Matthieu Allez, Larissa Nogueira Almeida, Giorgia Alvisi, Graham Anderson, Immanuel Andrä, Francesco Annunziato, Achille Anselmo, Petra Bacher, Cosima T. Baldari, Sudipto Bari, Vincenzo Barnaba, Joana Barros-Martins, Luca Battistini, Wolfgang Bauer, Sabine Baumgart, Nicole Baumgarth, Dirk Baumjohann, Bianka Baying, Mary Bebawy, Burkhard Becher, Wolfgang Beisker, Vladimir Benes, Rudi Beyaert, Alfonso Blanco, Dominic A. Boardman, Christian Bogdan, Jessica G. Borger, Giovanna Borsellino, Philip E. Boulais, Jolene A. Bradford, Dirk Brenner, Ryan R. Brinkman, Anna E.S. Brooks, Dirk H. Busch, Martin Büscher, Timothy P. Bushnell, Federica Calzetti, Garth Cameron, Ilenia Cammarata, Xuetao Cao, Susanna L. Cardell, Stefano Casola, Marco A. Cassatella, Andrea Cavani, Antonio Celada, Lucienne Chatenoud, Pratip K. Chattopadhyay, Sue Chow, Eleni Christakou, Luka Čičin-Šain, Mario Clerici, Federico S. Colombo, Laura Cook, Anne Cooke, Andrea M. Cooper, Alexandra J. Corbett, Antonio Cosma, Lorenzo Cosmi, Pierre G. Coulie, Ana Cumano, Ljiljana Cvetkovic, Van Duc Dang, Chantip Dang-Heine, Martin S. Davey, Derek Davies, Sara De Biasi, Genny Del Zotto, Gelo Victoriano Dela Cruz, Michael Delacher, Silvia Della Bella, Paolo Dellabona, Günnur Deniz, Mark Dessing, James P. Di Santo, Andreas Diefenbach, Francesco Dieli, Andreas Dolf, Thomas Dörner, Regine J. Dress, Diana Dudziak, Michael Dustin, Charles Antoine Dutertre, Friederike Ebner, Sidonia B.G. Eckle, Matthias Edinger, Pascale Eede, Götz R.A. Ehrhardt, Marcus Eich, Pablo Engel, Britta Engelhardt, Anna Erdei Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology. Vol.49, No.10 (2019), 1457-1973. doi:10.1002/eji.201970107 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/51016
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Title
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Author(s)
Andrea Cossarizza
Hyun Dong Chang
Andreas Radbruch
Andreas Acs
Dieter Adam
Sabine Adam-Klages
William W. Agace
Nima Aghaeepour
Mübeccel Akdis
Matthieu Allez
Larissa Nogueira Almeida
Giorgia Alvisi
Graham Anderson
Immanuel Andrä
Francesco Annunziato
Achille Anselmo
Petra Bacher
Cosima T. Baldari
Sudipto Bari
Vincenzo Barnaba
Joana Barros-Martins
Luca Battistini
Wolfgang Bauer
Sabine Baumgart
Nicole Baumgarth
Dirk Baumjohann
Bianka Baying
Mary Bebawy
Burkhard Becher
Wolfgang Beisker
Vladimir Benes
Rudi Beyaert
Alfonso Blanco
Dominic A. Boardman
Christian Bogdan
Jessica G. Borger
Giovanna Borsellino
Philip E. Boulais
Jolene A. Bradford
Dirk Brenner
Ryan R. Brinkman
Anna E.S. Brooks
Dirk H. Busch
Martin Büscher
Timothy P. Bushnell
Federica Calzetti
Garth Cameron
Ilenia Cammarata
Xuetao Cao
Susanna L. Cardell
Stefano Casola
Marco A. Cassatella
Andrea Cavani
Antonio Celada
Lucienne Chatenoud
Pratip K. Chattopadhyay
Sue Chow
Eleni Christakou
Luka Čičin-Šain
Mario Clerici
Federico S. Colombo
Laura Cook
Anne Cooke
Andrea M. Cooper
Alexandra J. Corbett
Antonio Cosma
Lorenzo Cosmi
Pierre G. Coulie
Ana Cumano
Ljiljana Cvetkovic
Van Duc Dang
Chantip Dang-Heine
Martin S. Davey
Derek Davies
Sara De Biasi
Genny Del Zotto
Gelo Victoriano Dela Cruz
Michael Delacher
Silvia Della Bella
Paolo Dellabona
Günnur Deniz
Mark Dessing
James P. Di Santo
Andreas Diefenbach
Francesco Dieli
Andreas Dolf
Thomas Dörner
Regine J. Dress
Diana Dudziak
Michael Dustin
Charles Antoine Dutertre
Friederike Ebner
Sidonia B.G. Eckle
Matthias Edinger
Pascale Eede
Götz R.A. Ehrhardt
Marcus Eich
Pablo Engel
Britta Engelhardt
Anna Erdei
Hyun Dong Chang
Andreas Radbruch
Andreas Acs
Dieter Adam
Sabine Adam-Klages
William W. Agace
Nima Aghaeepour
Mübeccel Akdis
Matthieu Allez
Larissa Nogueira Almeida
Giorgia Alvisi
Graham Anderson
Immanuel Andrä
Francesco Annunziato
Achille Anselmo
Petra Bacher
Cosima T. Baldari
Sudipto Bari
Vincenzo Barnaba
Joana Barros-Martins
Luca Battistini
Wolfgang Bauer
Sabine Baumgart
Nicole Baumgarth
Dirk Baumjohann
Bianka Baying
Mary Bebawy
Burkhard Becher
Wolfgang Beisker
Vladimir Benes
Rudi Beyaert
Alfonso Blanco
Dominic A. Boardman
Christian Bogdan
Jessica G. Borger
Giovanna Borsellino
Philip E. Boulais
Jolene A. Bradford
Dirk Brenner
Ryan R. Brinkman
Anna E.S. Brooks
Dirk H. Busch
Martin Büscher
Timothy P. Bushnell
Federica Calzetti
Garth Cameron
Ilenia Cammarata
Xuetao Cao
Susanna L. Cardell
Stefano Casola
Marco A. Cassatella
Andrea Cavani
Antonio Celada
Lucienne Chatenoud
Pratip K. Chattopadhyay
Sue Chow
Eleni Christakou
Luka Čičin-Šain
Mario Clerici
Federico S. Colombo
Laura Cook
Anne Cooke
Andrea M. Cooper
Alexandra J. Corbett
Antonio Cosma
Lorenzo Cosmi
Pierre G. Coulie
Ana Cumano
Ljiljana Cvetkovic
Van Duc Dang
Chantip Dang-Heine
Martin S. Davey
Derek Davies
Sara De Biasi
Genny Del Zotto
Gelo Victoriano Dela Cruz
Michael Delacher
Silvia Della Bella
Paolo Dellabona
Günnur Deniz
Mark Dessing
James P. Di Santo
Andreas Diefenbach
Francesco Dieli
Andreas Dolf
Thomas Dörner
Regine J. Dress
Diana Dudziak
Michael Dustin
Charles Antoine Dutertre
Friederike Ebner
Sidonia B.G. Eckle
Matthias Edinger
Pascale Eede
Götz R.A. Ehrhardt
Marcus Eich
Pablo Engel
Britta Engelhardt
Anna Erdei
Other Contributor(s)
Institute for Advanced Study of Technical University of Munich
Leibniz-Gemeinschaft
University of Auckland, School of Biological Sciences
A-Star, Singapore Immunology Network
The Francis Crick Institute
Humanitas University
Institut Pasteur - Fondazione Cenci Bolognetti
Istituto Italiano di Tecnologia
Duke-NUS Medical School Singapore
Fondazione IFOM Istituto Firc di Oncologia Molecolare
Fondazione Don Carlo Gnocchi
IRCCS San Raffaele Scientific Institute
Sony Europe Limited, Germany
Miltenyi Biotec
University of Luxembourg
Luxembourg Institute of Health
de Duve Institute
Theodor Kocher Institute of University of Bern
Universiteit Gent
University of Leicester
Thermo Fisher Scientific Inc.
Università degli Studi di Roma La Sapienza
Università degli Studi di Verona
NYU Langone Medical Center
National Cancer Centre, Singapore
University of Cambridge
Københavns Universitet
Universität Regensburg
Freie Universität Berlin
Università degli Studi di Milano
Universitätsklinik Erlangen und Medizinische Fakultät
Lunds Universitet
Universite Paris Descartes
Ludwig-Maximilians-Universität München
Istanbul Üniversitesi
Princess Margaret Cancer Centre
University of Melbourne
Charité – Universitätsmedizin Berlin
Eötvös Loránd University
Humanitas Research Hospital
University of Rochester Medical Center
Université Paris-Sud
Medizinische Hochschule Hannover (MHH)
Helmholtz Center Munich German Research Center for Environmental Health
University of Technology Sydney
Università degli Studi di Firenze
Klinikum der Universität Regensburg und Medizinische Fakultät
Technical University of Munich
University of Birmingham
Monash University
Nankai University
Università degli Studi di Palermo
University of Toronto
Göteborgs Universitet
University Hospital Zurich Institute of Experimental Immunology
IRCCS Istituto Giannina Gaslini - Ospedale Pediatrico
Odense Universitetshospital
University of California, Davis
IRCCS Fondazione Santa Lucia
Stanford University
University of Zurich
Universität zu Lübeck
Christian-Albrechts-Universität zu Kiel
University of Milano - Bicocca
Helmholtz Centre for Infection Research (HZI)
King's College London
Medizinische Universitat Wien
Danmarks Tekniske Universitet
The University of British Columbia
Istituto Superiore Di Sanita
BC Children's Hospital
Universität Bonn
European Molecular Biology Laboratory Heidelberg
Kennedy Institute of Rheumatology
Universitätsklinikum Schleswig-Holstein Campus Kiel
University College Dublin
Università degli Studi di Modena e Reggio Emilia
Terry Fox Laboratory
Università degli Studi di Siena
Albert Einstein College of Medicine of Yeshiva University
Institut Pasteur, Paris
Universitat de Barcelona
Friedrich-Alexander-Universität Erlangen-Nürnberg
German Center for Infection Research (DZIF)
Berlin Institute of Health (BIH)
Comprehensive Cancer Center Zurich
Regensburg Center for Interventional Immunology (RCI)
Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)
Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research
Leibniz-Gemeinschaft
University of Auckland, School of Biological Sciences
A-Star, Singapore Immunology Network
The Francis Crick Institute
Humanitas University
Institut Pasteur - Fondazione Cenci Bolognetti
Istituto Italiano di Tecnologia
Duke-NUS Medical School Singapore
Fondazione IFOM Istituto Firc di Oncologia Molecolare
Fondazione Don Carlo Gnocchi
IRCCS San Raffaele Scientific Institute
Sony Europe Limited, Germany
Miltenyi Biotec
University of Luxembourg
Luxembourg Institute of Health
de Duve Institute
Theodor Kocher Institute of University of Bern
Universiteit Gent
University of Leicester
Thermo Fisher Scientific Inc.
Università degli Studi di Roma La Sapienza
Università degli Studi di Verona
NYU Langone Medical Center
National Cancer Centre, Singapore
University of Cambridge
Københavns Universitet
Universität Regensburg
Freie Universität Berlin
Università degli Studi di Milano
Universitätsklinik Erlangen und Medizinische Fakultät
Lunds Universitet
Universite Paris Descartes
Ludwig-Maximilians-Universität München
Istanbul Üniversitesi
Princess Margaret Cancer Centre
University of Melbourne
Charité – Universitätsmedizin Berlin
Eötvös Loránd University
Humanitas Research Hospital
University of Rochester Medical Center
Université Paris-Sud
Medizinische Hochschule Hannover (MHH)
Helmholtz Center Munich German Research Center for Environmental Health
University of Technology Sydney
Università degli Studi di Firenze
Klinikum der Universität Regensburg und Medizinische Fakultät
Technical University of Munich
University of Birmingham
Monash University
Nankai University
Università degli Studi di Palermo
University of Toronto
Göteborgs Universitet
University Hospital Zurich Institute of Experimental Immunology
IRCCS Istituto Giannina Gaslini - Ospedale Pediatrico
Odense Universitetshospital
University of California, Davis
IRCCS Fondazione Santa Lucia
Stanford University
University of Zurich
Universität zu Lübeck
Christian-Albrechts-Universität zu Kiel
University of Milano - Bicocca
Helmholtz Centre for Infection Research (HZI)
King's College London
Medizinische Universitat Wien
Danmarks Tekniske Universitet
The University of British Columbia
Istituto Superiore Di Sanita
BC Children's Hospital
Universität Bonn
European Molecular Biology Laboratory Heidelberg
Kennedy Institute of Rheumatology
Universitätsklinikum Schleswig-Holstein Campus Kiel
University College Dublin
Università degli Studi di Modena e Reggio Emilia
Terry Fox Laboratory
Università degli Studi di Siena
Albert Einstein College of Medicine of Yeshiva University
Institut Pasteur, Paris
Universitat de Barcelona
Friedrich-Alexander-Universität Erlangen-Nürnberg
German Center for Infection Research (DZIF)
Berlin Institute of Health (BIH)
Comprehensive Cancer Center Zurich
Regensburg Center for Interventional Immunology (RCI)
Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)
Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research
Abstract
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.