Publication:
Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens

1

Issued Date

2007-02-01

Resource Type

Article

ISSN

00951137

DOI

10.1128/JCM.01823-06

Other identifier(s)

2-s2.0-33847014756

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Clinical Microbiology. Vol.45, No.2 (2007), 581-583

Suggested Citation

Kwannan Nantavisai, Mathirut Mungthin, Peerapan Tan-Ariya, Ram Rangsin, Tawee Naaglor, Saovanee Leelayoova Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens. Journal of Clinical Microbiology. Vol.45, No.2 (2007), 581-583. doi:10.1128/JCM.01823-06 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24584

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Title

Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens

Author(s)

Kwannan Nantavisai
 Mathirut Mungthin
 Peerapan Tan-Ariya
 Ram Rangsin
 Tawee Naaglor
 Saovanee Leelayoova

Other Contributor(s)

Mahidol University
 Phramongkutklao College of Medicine

Abstract

Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitivities and specificities. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Keyword(s)

Immunology and Microbiology
 Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/24584

Collections

Scopus 2006-2010