Publication: Involvement of the endocannabinoid system in periodontal healing
Issued Date
2010-04-16
Resource Type
ISSN
10902104
0006291X
0006291X
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2-s2.0-77950862353
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.394, No.4 (2010), 928-933
Suggested Citation
Sayaka Kozono, Takashi Matsuyama, Kamal Krishna Biwasa, Ko ichi Kawahara, Yumiko Nakajima, Takehiko Yoshimoto, Yutaka Yonamine, Hideshi Kadomatsu, Salunya Tancharoen, Teruto Hashiguchi, Kazuyuki Noguchi, Ikuro Maruyama Involvement of the endocannabinoid system in periodontal healing. Biochemical and Biophysical Research Communications. Vol.394, No.4 (2010), 928-933. doi:10.1016/j.bbrc.2010.03.080 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28737
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Title
Involvement of the endocannabinoid system in periodontal healing
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Abstract
Endocannabinoids including anandamide (AEA) and 2-arachidonoylglycerol (2-AG) are important lipid mediators for immunosuppressive effects and for appropriate homeostasis via their G-protein-coupled cannabinoid (CB) receptors in mammalian organs and tissues, and may be involved in wound healing in some organs. The physiological roles of endocannabinoids in periodontal healing remain unknown. We observed upregulation of the expression of CB1/CB2 receptors localized on fibroblasts and macrophage-like cells in granulation tissue during wound healing in a wound-healing model in rats, as well as an increase in AEA levels in gingival crevicular fluid after periodontal surgery in human patients with periodontitis. In-vitro, the proliferation of human gingival fibroblasts (HGFs) by AEA was significantly attenuated by AM251 and AM630, which are selective antagonists of CB1 and CB2, respectively. CP55940 (CB1/CB2 agonist) induced phosphorylation of the extracellular-regulated kinases (ERK) 1/2, p38 mitogen-activated protein kinase (p38MAPK), and Akt in HGFs. Wound closure by CP55940 in an in-vitro scratch assay was significantly suppressed by inhibitors of MAP kinase kinase (MEK), p38MAPK, and phosphoinositol 3-kinase (PI3-K). These findings suggest that endocannabinoid system may have an important role in periodontal healing. © 2010 Elsevier Inc. All rights reserved.