Publication: Differentiation between non-virulent and virulent Burkholderia pseudomallei with monoclonal antibodies to the Ara<sup>+</sup>or Ara<sup>-</sup>biotypes
Issued Date
2001-01-01
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ISSN
00029637
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2-s2.0-0034907763
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.65, No.1 (2001), 10-12
Suggested Citation
C. Thepthai, T. Dharakul, S. Smithikarn, S. Trakulsomboon, S. Songsivilai Differentiation between non-virulent and virulent Burkholderia pseudomallei with monoclonal antibodies to the Ara<sup>+</sup>or Ara<sup>-</sup>biotypes. American Journal of Tropical Medicine and Hygiene. Vol.65, No.1 (2001), 10-12. doi:10.4269/ajtmh.2001.65.10 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/26577
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Title
Differentiation between non-virulent and virulent Burkholderia pseudomallei with monoclonal antibodies to the Ara<sup>+</sup>or Ara<sup>-</sup>biotypes
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Abstract
Burkholderia pseudomallei is the causative agent of melioidosis, a fatal tropical infectious disease endemic in Southeast Asia. Environmental isolates of B. pseudomallei have two distinctive biotypes. Some soil isolates are arabinose-assimilators (Ara+biotype) and are non-virulent in experimental animals. The others cannot assimilate arabinose (Ara-biotype) and are virulent in experimental animals. The Ara-biotype is found in almost all B. pseudomallei clinical isolates. In the present study, a panel of eight monoclonal antibodies that agglutinate the bacteria were produced and tested. The first group, Bps-D2, -D3, -D5, -L1, and -L2 agglutinated 100% of Ara+clinical and soil isolates of B. pseudomallei. Another group Bps-A1, -A2, and -D1 agglutinated 92.9% and 90.9% of Ara-clinical and soil isolates, respectively. This panel of monoclonal antibodies may be useful for rapid differentiation between non-virulent Ara+and virulent Ara-B. pseudomallei.