Publication: HPLC analysis of stemokerrine and oxystemokerrine in the thai medicinal plant Stemona kerrii
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Issued Date
2012-11-29
Resource Type
ISSN
15559475
1934578X
1934578X
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2-s2.0-84869774131
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Mahidol University
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SCOPUS
Bibliographic Citation
Natural Product Communications. Vol.7, No.10 (2012), 1277-1278
Suggested Citation
Sumet Kongkiatpaiboon, Vichien Keeratinijakal, Wandee Gritsanapan HPLC analysis of stemokerrine and oxystemokerrine in the thai medicinal plant Stemona kerrii. Natural Product Communications. Vol.7, No.10 (2012), 1277-1278. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13367
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Title
HPLC analysis of stemokerrine and oxystemokerrine in the thai medicinal plant Stemona kerrii
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Abstract
A HPLC-UV method was developed and validated for determination of stemokerrine and oxystemokerrine in Stemona kerrii roots. The chromatographic separation was performed on a Hypersil BDS C 18 -column eluted with methanol: 50 mM ammonium acetate solution using a gradient system with a flow rate of 1 mL/min and detection at 300 nm. Stemokerrine and oxystemokerrine showed a linear relationship within the range of 0.5-100 μg/mL. The method was shown to be precise with a RSD < 2%. The average percent recovery of stemokerrine was 101.6% and for oxystemokerrine 99.5%. Two samples of S. kerrii were analyzed and the average contents of stemokerrine and oxystemokerrine were 0.2 and 0.05%, w/w, respectively. The present work will provide a useful standardization method for S. kerrii raw materials for further pharmaceutical development.