Publication: Chemometrics-assisted cross injection analysis for simultaneous determination of phosphate and silicate
Issued Date
2016-07-14
Resource Type
ISSN
10290397
03067319
03067319
Other identifier(s)
2-s2.0-84979223184
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Environmental Analytical Chemistry. Vol.96, No.9 (2016), 886-903
Suggested Citation
Kanchana Uraisin, Supavita Janya, Chutima Phechkrajang, Nathawut Choengchan, Warawut Tiyapongpattana, Víctor Cerdà, Duangjai Nacapricha Chemometrics-assisted cross injection analysis for simultaneous determination of phosphate and silicate. International Journal of Environmental Analytical Chemistry. Vol.96, No.9 (2016), 886-903. doi:10.1080/03067319.2016.1209664 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/40593
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Title
Chemometrics-assisted cross injection analysis for simultaneous determination of phosphate and silicate
Abstract
© 2016 Informa UK Limited, trading as Taylor & Francis Group. This work presents a novel method for simultaneous spectrophotometric determination of phosphate and silicate by using a cross injection analysis (CIA) coupled with the use of partial least squares (PLS) for data evaluation. The detection principle is based on the well-known ‘molybdenum blue’ method. The molybdate ions in the presence of stannous chloride in acidic medium give phosphomolybdenum blue and silicomolybdenum blue as products. In this work, all the liquids, including sample and reagents were simultaneously introduced into a CIA platform by using two peristaltic pumps for controlling the x-channel and y-channel flow which was automatically manipulated by using in-house control board. Crossflow provides sufficient mixing inside the platform prior detection of the absorption spectra of blue complexes in the wavelength of 400–900 nm. Since spectra of the blue colour product of phosphate and silicate are resemblant, these two analytes therefore reciprocally interfere with one another. This results in difficulty in simultaneous analysis of phosphate and silicate. In this work, PLS was utilised as assistor of CIA system for simultaneous analysis of phosphate and silicate using molybdenum blue reaction without using any modification of reagents and addition of selective masking agent. The calibration ranges are 0.1–6 mgP L−1 and 5–100 mgSi L−1 for phosphate and silicate, respectively. By using CIA coupled with PLS for data evaluation, the analysis of two analytes was achieved within 1.5 min with only single injection. The developed system was applied to natural water samples and the system was validated with the conventional methods. By statistical paired t-test, there was no evidence of significant difference at 95% confidence level (tstat = 2.28, tcritical = 2.31 and tstat = 0.62, tcritical = 2.31 for phosphate and silicate, respectively). This implied that the chemometrics-assisted CIA system was successfully developed for simultaneous spectrophotometric determination of phosphate and silicate.