Publication: Pueraria mirifica, phytoestrogen-induced change in synaptophysin expression via estrogen receptor in rat hippocampal neuron
Issued Date
2008-02-01
Resource Type
ISSN
01252208
01252208
01252208
Other identifier(s)
2-s2.0-40949139682
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.91, No.2 (2008), 208-214
Suggested Citation
Rattiya Chindewa, Saitida Lapanantasin, Yupin Sanvarinda, Sukumal Chongthammakun Pueraria mirifica, phytoestrogen-induced change in synaptophysin expression via estrogen receptor in rat hippocampal neuron. Journal of the Medical Association of Thailand. Vol.91, No.2 (2008), 208-214. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19763
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Pueraria mirifica, phytoestrogen-induced change in synaptophysin expression via estrogen receptor in rat hippocampal neuron
Other Contributor(s)
Abstract
Objective: To examine Pueraria mirifica (Leguminosae) containing- phytoestrogen effect on synaptic density and involvement of estrogen receptor. Material and Method: The level of synaptophysin, a presynaptic vesicle protein, was measured using Western blot analysis and immunocytochemistry in hippocampal primary cell cultures at 6 days in vitro. Results: P. mirifica and 17?-estradiol (0.1 ?M) treatment for 4 days, but not for 2 days, significantly increased synaptophysin immunoreactivity and level of synaptophysin. P. mirifica up to 60 ?g/ml resulted in a dose related increase in the level of synaptophysin immunoreactivity. The classical estrogen receptor antagonist, ICI 182 780, significantly blocked P. mirifica-induced increase in synaptophysin. Conclusion: P. mirifica-containing phytoestrogen affects synaptic density by inducing synaptophysin expression via estrogen receptor.