Publication: Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules
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Issued Date
2011-11-01
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ISSN
1769714X
12864579
12864579
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2-s2.0-80053386594
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Mahidol University
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SCOPUS
Bibliographic Citation
Microbes and Infection. Vol.13, No.12-13 (2011), 1006-1011
Suggested Citation
Supaporn Suparak, Veerachat Muangsombut, Donporn Riyapa, Joanne M. Stevens, Mark P. Stevens, Ganjana Lertmemongkolchai, Sunee Korbsrisate Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules. Microbes and Infection. Vol.13, No.12-13 (2011), 1006-1011. doi:10.1016/j.micinf.2011.06.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11973
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Title
Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules
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Abstract
Burkholderia pseudomallei induces the formation of multinucleated giant cells in cell monolayers. After infection of human macrophage-like U937 cells with B. pseudomallei, addition of monoclonal antibodies against integrin-associated protein (CD47), E-selectin (CD62E), a fusion regulatory protein (CD98), and E-cadherin (CD324) suppressed multinucleated giant cells in a concentration-dependent manner while monoclonal antibodies against other surface molecules did not inhibit fusion despite binding to the cell surface. Flow cytometric analysis showed increased expression of CD47 and CD98, but not CD62E and CD324, upon B. pseudomallei infection. Our data suggest the involvement of specific cellular factors in the process of B. pseudomallei-induced fusion. © 2011 Institut Pasteur.