Publication: Geographic distribution of amino acid mutations in DHFR and DHPS in Plasmodium vivax isolates from Lao PDR, India and Colombia
Issued Date
2016
Resource Type
Language
eng
Rights
Mahidol University
Rights Holder(s)
BioMed Central
Bibliographic Citation
Malaria Journal. Vol.15, (2016), 484
Suggested Citation
Naowarat Saralamba, Supatchara Nakeesathit, Mayfong Mayxay, Newton, Paul N., Lyda Osorio, Kim, Jung‑Ryong, White, Nicholas J., Day, Nicholas P. J., Dondorp, Arjen M., Mallika Imwong Geographic distribution of amino acid mutations in DHFR and DHPS in Plasmodium vivax isolates from Lao PDR, India and Colombia. Malaria Journal. Vol.15, (2016), 484. doi:10.1186/s12936-016-1543-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/3160
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Geographic distribution of amino acid mutations in DHFR and DHPS in Plasmodium vivax isolates from Lao PDR, India and Colombia
Abstract
Background: Non-synonymous mutations in dhfr and dhps genes in Plasmodium vivax are associated with sulfadoxine–
pyrimethamine (SP) resistance. The present study aimed to assess the prevalence of point mutations in P. vivax
dhfr (pvdhfr) and P. vivax dhps (pvdhps) genes in three countries: Lao PDR, India and Colombia.
Methods: Samples from 203 microscopically diagnosed vivax malaria were collected from the three countries. Five
codons at positions 13, 57, 58, 61, and 117 of pvdhfr and two codons at positions 383 and 553 of pvdhps were examined
by polymerase chain reaction-restriction fragment length polymorphism methodology.
Results: The largest number of 58R/117 N double mutations in pvdhfr was observed in Colombia (94.3 %), while the
corresponding wild-type amino acids were found at high frequencies in Lao PDR during 2001–2004 (57.8 %). Size
polymorphism analysis of the tandem repeats within pvdhfr revealed that 74.3 % of all the isolates carried the type B
variant. Eighty-nine per cent of all the isolates examined carried wild-type pvdhps A383 and A553.
Conclusions: Although SP is not generally used to treat P. vivax infections, mutations in dhfr and dhps that confer
antifolate resistance in P. vivax are common. The data strongly suggest that, when used primarily to treat falciparum
malaria, SP can exert a substantial selective pressure on P. vivax populations, and this can lead to point mutations in
dhfr and dhps. Accurate data on the global geographic distribution of dhfr and dhps genotypes should help to inform
anti-malarial drug-use policies.