Publication: Simultaneous determination of paracetamol and chlorpheniramine maleate by micellar electrokinetic chromatography
3
Issued Date
2003-10-15
Resource Type
ISSN
07317085
Other identifier(s)
2-s2.0-0141433446
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Pharmaceutical and Biomedical Analysis. Vol.33, No.3 (2003), 441-449
Suggested Citation
Leena Suntornsuk, Ongart Pipitharome, Prapin Wilairat Simultaneous determination of paracetamol and chlorpheniramine maleate by micellar electrokinetic chromatography. Journal of Pharmaceutical and Biomedical Analysis. Vol.33, No.3 (2003), 441-449. doi:10.1016/S0731-7085(03)00288-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20686
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Simultaneous determination of paracetamol and chlorpheniramine maleate by micellar electrokinetic chromatography
Other Contributor(s)
Abstract
A micellar electrokinetic chromatography (MEKC) method was established for determination of paracetamol (PARA) and chlorpheniramine maleate (CPM) in cold tablets. Separation of both drugs, as well as other seven cold remedy ingredients, was achieved in 25.5 min using a sodium dihydrogenphosphate-sodium tetraborate buffer (10 mM, pH 9.0) containing sodium dodecyl sulfate (SDS) (50 mM) and acetonitrile (26% v/v). The effective capillary length of 50 cm, the separating voltage of 15 kV and the temperature of 30°C was optimized. Detection was by a diode array detector at 214 nm. Method linearity was excellent (r2>0.999) over the concentration tested (10-250 μg/ml) with good precision and accuracy. Recoveries were good (>99%) with limits of detection of 0.4 and 0.5 μg/ml and limits of quantitation of 2 (%R.S.D.=3.1%) and 4 (%R.S.D.=2.4%) μg/ml, for PARA and CPM, respectively. The developed method was applied to the determination of ingredients in cold tablets and was found to be simple, rapid and efficient. © 2003 Elsevier B.V. All rights reserved.