Publication: Cytotoxic and the proliferative effect of cuttlefish bone on MC3T3-E1 osteoblast cell line
Issued Date
2017-10-01
Resource Type
ISSN
13057464
13057456
13057456
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2-s2.0-85042219449
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Mahidol University
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SCOPUS
Bibliographic Citation
European Journal of Dentistry. Vol.11, No.4 (2017), 503-507
Suggested Citation
La Ongthong Vajrabhaya, Suwanna Korsuwannawong, Rudee Surarit Cytotoxic and the proliferative effect of cuttlefish bone on MC3T3-E1 osteoblast cell line. European Journal of Dentistry. Vol.11, No.4 (2017), 503-507. doi:10.4103/ejd.ejd_159_17 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42429
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Title
Cytotoxic and the proliferative effect of cuttlefish bone on MC3T3-E1 osteoblast cell line
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Abstract
© 2017 European Journal of Dentistry. Objective: To evaluate the cytotoxic and the proliferative effect of cuttlefish bone on MC3T3-E1 osteoblast cell line. Materials and Methods: MC3T3-E1 cells were treated with 0.5, 1, 5, 25, 50, 100, or 200 μg/ml cuttlefish bone powder (CBP). Cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. This assay was also used to determine cell proliferation over 16 days of treatment with 0.5, 25, or 100 μg/ml CBP. Results: CBP was not cytotoxic to MC3T3-E1 cells at any concentration. The percentage of cell viability in the 0.5-200 μg/ml CBP groups dose dependently decreased from 107.52 ± 11.03 to 92.48 ± 5.60%; however, the differences between the groups or the negative control group were not significant. At 16 days, 0.5, 25, and 100 μg/ml CBP groups showed 123.19 ± 10.07%, 126.02 ± 15.69%, and 133.33 ± 11.74% proliferation, respectively, that were significantly higher than that of the control group. Conclusion: These results indicate that CBP promotes osteoblast proliferation and may be a potential material to increase the number of osteoblasts in a bone defect in the oral cavity.