Novel DNA amplification on chromosomes 6q23-24 and 4p15.2 in breast cancer identified by arbitrarily primed polymerase chain reaction

Abstract

Breast cancer is the most common cancer among women worldwide. The molecular basis of breast cancer has not yet been fully elucidated. In this report, novel DNA amplification on chromosomes 6q23-24 and 4p15.2 were identified by arbitrarily primed polymerase chain reaction, gene cloning, nucleotide sequencing and identified by comparison with known sequences in genome data base, and quantitated by real-time PCR. Results revealed that 25 of 32 (78.1%) breast cancer cases harbored DNA amplification on chromosomes 6q23-24 and 4p15.2. There was a significant association between increase in tumor size (>3cm) and DNA amplification on chromosome 6q23-24 (Odds ratio = 13.75, 95% CI = 1.26-350.38, P = 0.018). The results indicated that DNA amplification on chromosome 6q23-24 may be involved in the progression of breast cancer.