Cytogenetic and Hybridization Studies among 3 Strains Morphologically variated and belonging to Anopheles minimus Theobald from Japan and Thailand

Abstract

In genetic studies on Anopheles minimus cytogenetic observations and hybridization tests were performed on 3 strains belonging to Anopheles minimus and their hybrids, in order to know cytotaxonomic informations and phylogenetic relationships among geographical populations of the species due to their morphological varieties. The results were revealed their homosequencial cytomorphology, especially in their banding sequencies of polytene chromosomes of both salivary glands and ovarian nurce cells. No naturally occurring polymorphism was described from the observations of each 50 slides of the 3 strains, one from Ishigaki Island, Japan other 2 from Kanchanaburi, Thailand; relating to them morphological varieties were also qualified among them. Complete synaptic salivary gland polytene chromosomes were observed among their F1 hybrids from 3 combinations of crosses among the strains. These chromosomal informations gave standarized polytene chromosome map and its description was made. Hybridization experiment among the 3 strains revealed some grades of postcoupuratory reproductive isolation-phenomenon between ISG strain and KCH-1 strain whereas not between ISG and KCH-2 one. The one was inviability of the F1 hybrid from reciprocal crosses between ISG and KCH-1. © 1984, Japan Mendel Society, International Society of Cytology. All rights reserved.