Publication: Prevalence and gB genotype distribution of human cytomegalovirus among HIV sero-negative and HIV sero-positive orphans in Thailand
Issued Date
2014-01-01
Resource Type
ISSN
01252208
01252208
01252208
Other identifier(s)
2-s2.0-84929624785
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.97, (2014), S136-S144
Suggested Citation
Rungnapa Hyatt, Peerapan Tan-Ariya, Saowakon Paca-Uccaralertkun, Mathirut Mungthin, Sureeporn Poungpong Prevalence and gB genotype distribution of human cytomegalovirus among HIV sero-negative and HIV sero-positive orphans in Thailand. Journal of the Medical Association of Thailand. Vol.97, (2014), S136-S144. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34491
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Prevalence and gB genotype distribution of human cytomegalovirus among HIV sero-negative and HIV sero-positive orphans in Thailand
Other Contributor(s)
Abstract
© 2014, Medical Association of Thailand. All Rights Reserved. Background: Human Cytomegalovirus (HCMV) infects humans in all geographic areas. Polymorphisms of glycoprotein B (gB) have been used for genotypic characterization of HCMV. However, information of gB genotyping of HCMV in Thailand is not clearly known especially in children. Material and Method: A cross-sectional study was conducted to assess HCMV infection in 236 HIV seronegative and HIV seropositive children who attended an orphanage in Nonthaburi, Thailand by nested-PCR technique using urine specimens. HCMV gB genotypes were determined by restriction fragment length polymorphism (RFLP), and DNA sequencing technique. Results: Sixty-one percent (144/236) of the samples were HCMV positive, which consisted of 66.1% (37/56) of the HIV seropositive children and 59.4% (107/180) of the HIV sero-negative children. Multivariate analysis showed that children who living in one particular room were independently associated with HCMV infection. Genotypic analysis revealed that the most prevalent genotype in these children was gB1; 85.4% (111/130) followed by gB3; 4.6% (6/130), gB2 and gB4 each at 2.3% (3/130). Mixed gB genotypes were identified in 5.4% (7/130) of the samples. Conclusion: HCMV infection, in particular gB1 genotype was commonly identified among these Thai orphans. Living in one particular room was associated with getting the infection. To prevent the transmission of HCMV infection in this setting, improvement in hygienic behavior of childcare workers should be focused.