Publication: Factors affecting salivary α-amylase levels measured with a handheld biosensor and a standard laboratory assay
Issued Date
2019-11-01
Resource Type
ISSN
15206300
10420533
10420533
Other identifier(s)
2-s2.0-85069719718
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Human Biology. Vol.31, No.6 (2019)
Suggested Citation
Kanlayanee Tangprasert, Nattinee Jantaratnotai, Praewpat Pachimsawat Factors affecting salivary α-amylase levels measured with a handheld biosensor and a standard laboratory assay. American Journal of Human Biology. Vol.31, No.6 (2019). doi:10.1002/ajhb.23298 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/49707
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Factors affecting salivary α-amylase levels measured with a handheld biosensor and a standard laboratory assay
Other Contributor(s)
Abstract
© 2019 Wiley Periodicals, Inc. Objectives: A handheld biosensor for measuring salivary α-amylase (sAA) was developed for convenient on-site measurement. Previous studies reported some discrepancies in sAA levels measured with a biosensor and a standard assay. This study aimed to compare sAA levels measured with three different methods and the factors affecting its levels. Methods: Thirty-eight participants collected saliva two times for three measurements. First, the collector strip was placed under the tongue for 2 minutes, then the strip was used to measure sAA level on-site immediately (intraoral biosensor; method 1). Then, a participant pooled the saliva for 4 minutes and collected the saliva into the tube which was aliquoted to measure in a laboratory with a handheld biosensor (extraoral biosensor; method 2) and with a standard enzyme kinetic assay (EKA; method 3). Additional experiments were carried out to compare the levels of sAA measured with differences in pooling time and positioning of the collector strip. Results: A high correlation of sAA levels between an extraoral and an EKA measurement (r = 0.989) was observed, while sAA levels measured with an intraoral method showed a significant but weaker correlation with either an EKA (r = 0.475) or an extraoral method (r = 0.436). Saliva pooling time and positioning of the collector strip significantly affected sAA levels. Conclusions: A handheld biosensor is valid to measure sAA levels extraorally. For an intraoral measurement, pooling time and positioning of the collector strip need to be taken into account.