Publication: Mass spectrometric analysis of host cell proteins interacting with dengue virus nonstructural protein 1 in dengue virus-infected HepG2 cells
Issued Date
2016-09-01
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ISSN
18781454
15709639
15709639
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2-s2.0-84966716417
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochimica et Biophysica Acta - Proteins and Proteomics. Vol.1864, No.9 (2016), 1270-1280
Suggested Citation
Thanyaporn Dechtawewat, Atchara Paemanee, Sittiruk Roytrakul, Pucharee Songprakhon, Thawornchai Limjindaporn, Pa thai Yenchitsomanus, Sawanan Saitornuang, Chunya Puttikhunt, Watchara Kasinrerk, Prida Malasit, Sansanee Noisakran Mass spectrometric analysis of host cell proteins interacting with dengue virus nonstructural protein 1 in dengue virus-infected HepG2 cells. Biochimica et Biophysica Acta - Proteins and Proteomics. Vol.1864, No.9 (2016), 1270-1280. doi:10.1016/j.bbapap.2016.04.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42974
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Title
Mass spectrometric analysis of host cell proteins interacting with dengue virus nonstructural protein 1 in dengue virus-infected HepG2 cells
Abstract
© 2016 Elsevier B.V. Dengue virus (DENV) infection is a leading cause of the mosquito-borne infectious diseases that affect humans worldwide. Virus–host interactions appear to play significant roles in DENV replication and the pathogenesis of DENV infection. Nonstructural protein 1 (NS1) of DENV is likely involved in these processes; however, its associations with host cell proteins in DENV infection remain unclear. In this study, we used a combination of techniques (immunoprecipitation, in-solution trypsin digestion, and LC–MS/MS) to identify the host cell proteins that interact with cell-associated NS1 in an in vitro model of DENV infection in the human hepatocyte HepG2 cell line. Thirty-six novel host cell proteins were identified as potential DENV NS1-interacting partners. A large number of these proteins had characteristic binding or catalytic activities, and were involved in cellular metabolism. Coimmunoprecipitation and colocalization assays confirmed the interactions of DENV NS1 and human NIMA-related kinase 2 (NEK2), thousand and one amino acid protein kinase 1 (TAO1), and component of oligomeric Golgi complex 1 (COG1) proteins in virus-infected cells. This study reports a novel set of DENV NS1-interacting host cell proteins in the HepG2 cell line and proposes possible roles for human NEK2, TAO1, and COG1 in DENV infection.