Publication: Cytotoxicity and histopathological analysis of titanium nanoparticles via Artemia salina
Issued Date
2019-05-01
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ISSN
16147499
09441344
09441344
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2-s2.0-85045743663
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Mahidol University
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SCOPUS
Bibliographic Citation
Environmental Science and Pollution Research. Vol.26, No.15 (2019), 14706-14711
Suggested Citation
Supicha Kachenton, Wannee Jiraungkoorskul, Niwat Kangwanrangsan, Tawewan Tansatit Cytotoxicity and histopathological analysis of titanium nanoparticles via Artemia salina. Environmental Science and Pollution Research. Vol.26, No.15 (2019), 14706-14711. doi:10.1007/s11356-018-1856-y Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50920
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Title
Cytotoxicity and histopathological analysis of titanium nanoparticles via Artemia salina
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Abstract
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. The consumption trend of nanoparticles by industry in this moment pays attention to titanium nanoparticles (TiNPs), due to their various applications: personal care products, household products, food industry, electronic devices, and healthcare products. Rising consumption of TiNPs without specific regulatory criteria for control safety releasing quantification leads to concern on the topic of environmental contamination and injurious effect. Therefore, this study investigates TiNP toxicities on aquatic animals representing hazardous effects to natural water resource, by determining 24-h LC50 of TiNPs with histopathology investigation. We select brine shrimp (Artemia salina) as a model. Ten adults A. salina were incubated at room temperature for 24 h with various concentrations of TiNPs in triplicate. The mortality number of A. salina was recorded and LC50 value was calculated. The LC50 result is 1693.43 mg/L. Next, A. salina histopathology investigation was done by selecting the living ones after incubation for 24 h with 25% LC50 of TiNPs. We performed tissue processing, embedding, sectioning, and H&E staining, and observed under light microscope. Histopathology reveals TiNP occlusion throughout the intestinal tract. Epithelial cells show abnormal morphology such as hyperplasia, villus deformation, disorganized arrangement, severe edema, and necrosis area. Consequently, the current study shows the severity of TiNP effects on aquatic microcrustaceans and their negative impact on the ecosystem. Furthermore, this information will aid the elucidation of TiNP toxicity effect and the risk of ecosystem disruptions.