Publication:
A suppressor of the menadione-hypersensitive phenotype of a Xanthomonas campestris pv. phaseoli oxyR mutant reveals a novel mechanism of toxicity and the protective role of alkyl hydroperoxide reductase

1

Issued Date

2003-03-01

Resource Type

Article

ISSN

00219193

DOI

10.1128/JB.185.5.1734-1738.2003

Other identifier(s)

2-s2.0-0037369807

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Bacteriology. Vol.185, No.5 (2003), 1734-1738

Suggested Citation

Paiboon Vattanaviboon, Wirongrong Whangsuk, Skorn Mongkolsuk A suppressor of the menadione-hypersensitive phenotype of a Xanthomonas campestris pv. phaseoli oxyR mutant reveals a novel mechanism of toxicity and the protective role of alkyl hydroperoxide reductase. Journal of Bacteriology. Vol.185, No.5 (2003), 1734-1738. doi:10.1128/JB.185.5.1734-1738.2003 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20915

Research Projects

Organizational Units

Authors

Journal Issue

Thesis

Title

A suppressor of the menadione-hypersensitive phenotype of a Xanthomonas campestris pv. phaseoli oxyR mutant reveals a novel mechanism of toxicity and the protective role of alkyl hydroperoxide reductase

Author(s)

Paiboon Vattanaviboon
 Wirongrong Whangsuk
 Skorn Mongkolsuk

Other Contributor(s)

Chulabhorn Research Institute
 Mahidol University

Abstract

We isolated menadione-resistant mutants of Xanthomonas campestris pv. phaseoli oxyR (oxyRXp). The oxyRR2Xp mutant was hyperresistant to the superoxide generators menadione and plumbagin and was moderately resistant to H2O2 and tert-butyl hydroperoxide. Analysis of enzymes involved in oxidative-stress protection in the oxyRR2Xp mutant revealed a >10-fold increase in AhpC and AhpF levels, while the levels of superoxide dismutase (SOD), catalase, and the organic hydroperoxide resistance protein (Ohr) were not significantly altered. Inactivation of ahpC in the oxyRR2Xp mutant resulted in increased sensitivity to menadione killing. Moreover, high levels of expression of cloned ahpC and ahpF in the oxyRXp mutant complemented the menadione hypersensitivity phenotype. High levels of other oxidant-scavenging enzymes such as catalase and SOD did not protect the cells from menadione toxicity. These data strongly suggest that the toxicity of superoxide generators could be mediated via organic peroxide production and that alkyl hydroperoxide reductase has an important novel function in the protection against the toxicity of these compounds in X. campestris.

Keyword(s)

Immunology and Microbiology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/20915

Collections

Scopus 2001-2005
Load more