Publication: Development of single-tube mutiplex RT-PCR for dengue virus typing
| dc.contributor.author | Pannamas Maneekan | en_US |
| dc.contributor.author | Akanitt Jittmittraphap | en_US |
| dc.contributor.author | Pornsawan Leaungwutiwong | en_US |
| dc.contributor.author | Jaranit Kaewkangwal | en_US |
| dc.contributor.author | Natthanej Luplertlop | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.date.accessioned | 2018-09-13T06:51:40Z | |
| dc.date.available | 2018-09-13T06:51:40Z | |
| dc.date.issued | 2009-11-01 | en_US |
| dc.description.abstract | This study evaluated a single-tube multiplex RT-PCR with a primer focusing on nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it reduces time and decreases risk of contamination. | en_US |
| dc.identifier.citation | Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.6 (2009), 1254-1258 | en_US |
| dc.identifier.issn | 01251562 | en_US |
| dc.identifier.other | 2-s2.0-76749114335 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/27864 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=76749114335&origin=inward | en_US |
| dc.subject | Medicine | en_US |
| dc.title | Development of single-tube mutiplex RT-PCR for dengue virus typing | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=76749114335&origin=inward | en_US |