Publication: Characterization of an organic-solvent-tolerant brevibacillus agri strain 13 able to stabilize solvent/water emulsion
Issued Date
2009-08-01
Resource Type
ISSN
15746968
03781097
03781097
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2-s2.0-68249156599
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Mahidol University
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SCOPUS
Bibliographic Citation
FEMS Microbiology Letters. Vol.297, No.2 (2009), 225-233
Suggested Citation
Ajiraporn Kongpol, Thunyarat Pongtharangkul, Junichi Kato, Kohsuke Honda, Hisao Ohtake, Alisa S. Vangnai Characterization of an organic-solvent-tolerant brevibacillus agri strain 13 able to stabilize solvent/water emulsion. FEMS Microbiology Letters. Vol.297, No.2 (2009), 225-233. doi:10.1111/j.1574-6968.2009.01684.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27172
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Title
Characterization of an organic-solvent-tolerant brevibacillus agri strain 13 able to stabilize solvent/water emulsion
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Abstract
Brevibacillus agri strain 13 was isolated and characterized as a Gram-positive organic-solvent-tolerant bacterium able to grow at 45 °C. It can tolerate high concentrations (5% and 20%, v/v) of various organic solvents with a broad range of log Powwhen the organic solvent was provided as a nonaqueous layer. Although it can tolerate a number of aromatic solvents, it cannot utilize them as a sole carbon source. The surface characteristics of cells exposed to organic solvent were investigated using the bacterial adhesion to hydrocarbon test, a contact angle measurement, ζ potential determination, and fluorescence microscopy analysis and compared with that of nonexposed cells. The results showed that although it has a hydrophilic cell surface, it has a unique indigenous cell surface characteristic in which the cells can stabilize solvent-in-water emulsion by adhering to the solvent-water interface of the solvent droplets. The tolerance and predilection of B. agri strain 13 toward organic solvents may suggest its potential application as a whole-cell biocatalyst for the biotransformation process of water-immiscible substrate(s). © 2009 Federation of European Microbiological Societies.