Publication:
Sensitivity and specificity of polymerase chain reaction for the detection of Toxoplasma gondii DNA added to laboratory samples

3

Issued Date

2005-03-01

Resource Type

Article

ISSN

01251562

Other identifier(s)

2-s2.0-20444485745

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.2 (2005), 408-411

Suggested Citation

Rachatawan Chiabchalard, Jitbanjong Toompong Wiengcharoen, Yaowalark Sukthana Sensitivity and specificity of polymerase chain reaction for the detection of Toxoplasma gondii DNA added to laboratory samples. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.2 (2005), 408-411. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/17026

Research Projects

Organizational Units

Authors

Journal Issue

Thesis

Title

Sensitivity and specificity of polymerase chain reaction for the detection of Toxoplasma gondii DNA added to laboratory samples

Author(s)

Rachatawan Chiabchalard
 Jitbanjong Toompong Wiengcharoen
 Yaowalark Sukthana

Other Contributor(s)

Mahidol University

Abstract

We studied the sensitivity and specificity of PCR to detect T.gondii DNA by aliquoting various concentration of tachyzoites into laboratory specimens from 60 positive and 10 negative buffy-coat samples. We were able to detect the specific gene from purified DNA samples containing as few as 0.25 parasites per 100,000 human leukocytes. These results had an impressive initial 100% specificity but later it decreased because of false-negative data.

Keyword(s)

Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/17026

Collections

Scopus 2001-2005