Publication:
Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against hiv-1

Issued Date

2021-09-01

Resource Type

Article

ISSN

2050084X

DOI

10.7554/eLife.69577

Other identifier(s)

2-s2.0-85116328012

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

eLife. Vol.10, (2021)

Suggested Citation

Shida Shangguan, Philip K. Ehrenberg, Aviva Geretz, Lauren Yum, Gautam Kundu, Kelly May, Slim Fourati, Krystelle Nganou-Makamdop, Latonya D. Williams, Sheetal Sawant, Eric Lewitus, Punnee Pitisuttithum, Sorachai Nitayaphan, Suwat Chariyalertsak, Supachai Rerks-Ngarm, Morgane Rolland, Daniel Douek, Peter Gilbert, Georgia D. Tomaras, Nelson Michael, Sandhya Vasan, Rasmi Thomas Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against hiv-1. eLife. Vol.10, (2021). doi:10.7554/eLife.69577 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76036

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Title

Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against hiv-1

Author(s)

Shida Shangguan
 Philip K. Ehrenberg
 Aviva Geretz
 Lauren Yum
 Gautam Kundu
 Kelly May
 Slim Fourati
 Krystelle Nganou-Makamdop
 Latonya D. Williams
 Sheetal Sawant
 Eric Lewitus
 Punnee Pitisuttithum
 Sorachai Nitayaphan
 Suwat Chariyalertsak
 Supachai Rerks-Ngarm
 Morgane Rolland
 Daniel Douek
 Peter Gilbert
 Georgia D. Tomaras
 Nelson Michael
 Sandhya Vasan
 Rasmi Thomas

Other Contributor(s)

Faculty of Tropical Medicine, Mahidol University
 Armed Forces Research Institute of Medical Sciences, Thailand
 Thailand Ministry of Public Health
 HJF
 Walter Reed Army Institute of Research
 National Institutes of Health (NIH)
 Duke University School of Medicine
 Fred Hutchinson Cancer Research Center
 Emory University
 Chiang Mai University

Abstract

A gene signature previously correlated with mosaic adenovirus 26 vaccine protection in simian immunodeficiency virus (SIV) and SHIV challenge models in non-human primates (NHP). In this report we investigated presence of this signature as a correlate of reduced risk in human clinical trials and potential mechanisms of protection. The absence of this gene signature in the DNA/rAd5 human vaccine trial, which did not show efficacy, strengthens our hypothesis that this signature is only enriched in studies that demonstrated protection. This gene signature was enriched in the partially effective RV144 human trial that administered the ALVAC/protein vaccine, and we find that the signature associates with both decreased risk of HIV-1 acquisition and increased vaccine efficacy. Total RNA-seq in a clinical trial that used the same vaccine regimen as the RV144 HIV vaccine implicated antibody-dependent cellular phagocytosis (ADCP) as a potential mechanism of vaccine protection. CITE-seq profiling of 53 surface markers and transcriptomes of 53,777 single cells from the same trial showed that genes in this signature were primarily expressed in cells belonging to the myeloid lineage, including monocytes, which are major effector cells for ADCP. The consistent association of this transcriptome signature with vaccine efficacy represents a tool both to identify potential mechanisms, as with ADCP here, and to screen novel approaches to accelerate development of new vaccine candidates.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Immunology and Microbiology
 Neuroscience

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/76036

Collections

Scopus 2021