Publication: Mutations of maturity-onset diabetes of the young (MODY) genes in Thais with early-onset type 2 diabetes mellitus: ORIGINAL ARTICLE
Issued Date
2009-06-01
Resource Type
ISSN
13652265
03000664
03000664
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2-s2.0-66149107461
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinical Endocrinology. Vol.70, No.6 (2009), 847-853
Suggested Citation
Nattachet Plengvidhya, Watip Boonyasrisawat, Nalinee Chongjaroen, Prapaporn Jungtrakoon, Sutin Sriussadaporn, Sathit Vannaseang, Napatawn Banchuin, Pa Thai Yenchitsomanus Mutations of maturity-onset diabetes of the young (MODY) genes in Thais with early-onset type 2 diabetes mellitus: ORIGINAL ARTICLE. Clinical Endocrinology. Vol.70, No.6 (2009), 847-853. doi:10.1111/j.1365-2265.2008.03397.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28061
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Title
Mutations of maturity-onset diabetes of the young (MODY) genes in Thais with early-onset type 2 diabetes mellitus: ORIGINAL ARTICLE
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Abstract
Objective Six known genes responsible for maturity-onset diabetes of the young (MODY) were analysed to evaluate the prevalence of their mutations in Thai patients with MODY and early-onset type 2 diabetes. Patients and methods Fifty-one unrelated probands with early-onset type 2 diabetes, 21 of them fitted into classic MODY criteria, were analysed for nucleotide variations in promoters, exons, and exon-intron boundaries of six known MODY genes, including HNF-4α, GCK, HNF-1α, IPF-1, HNF-1β, and NeuroD1/β2, by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method followed by direct DNA sequencing. Missense mutations or mutations located in regulatory region, which were absent in 130 chromosomes of non-diabetic controls, were classified as potentially pathogenic mutations. Results We found that mutations of the six known MODY genes account for a small proportion of classic MODY (19%) and early-onset type 2 diabetes (10%) in Thais. Five of these mutations are novel including GCK R327H, HNF-1α P475L, HNF-1α G554fsX556, NeuroD1 -1972 G > A and NeuroD1 A322N. Mutations of IPF-1 and HNF-1β were not identified in the studied probands. Conclusions Mutations of the six known MODY genes may not be a major cause of MODY and early-onset type 2 diabetes in Thais. Therefore, unidentified genes await discovery in a majority of Thai patients with MODY and early-onset type 2 diabetes. © 2009 Blackwell Publishing Ltd.