Publication: Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon
Issued Date
2017-06-01
Resource Type
ISSN
10959947
10504648
10504648
Other identifier(s)
2-s2.0-85017449346
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Mahidol University
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SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology. Vol.65, (2017), 88-95
Suggested Citation
Sittichai Boonrawd, Ravi Mani, Sirikwan Ponprateep, Premruethai Supungul, Promsin Masrinoul, Anchalee Tassanakajon, Vichien Rimphanitchayakit Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon. Fish and Shellfish Immunology. Vol.65, (2017), 88-95. doi:10.1016/j.fsi.2017.04.005 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41484
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Title
Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon
Abstract
© 2017 Elsevier Ltd Spätzle is a signaling ligand in innate immune response that signals pathogenic infection via Toll receptor and Toll pathway into the cells for the synthesis of antimicrobial proteins. Herein, three PmSpӓtzle isoforms were identified in Penaeus monodon, namely PmSpz1, 2 and 3. The PmSpz1 was chosen for detailed study. The PmSpz1 gene was expressed in all nine tissues tested including the hemocytes, stomach, hepatopancreas, gill, lymphoid tissue, eyestalk, muscle, intestine and heart. Its expression was up-regulated upon white spot syndrome virus (WSSV) infection. Western blot analysis of hemolymph showed that the PmSpz1 mostly existed as a cleaved active form awaiting to activate the Toll pathway. Injection of a recombinant PmSpz1 rendered the shrimp less susceptible to the WSSV infection. Injection of a recombinant active form of PmSpz1 into a normal shrimp activated the synthesis of crustinPm1, crustinPm7, ALFPm3, penaeidin3 but not penaeidin5 indicating that the expression of all antimicrobial proteins but not penaeidin5 was under the regulation of Toll pathway.