Publication: Cloning of cytochrome P450, CYP6P5, and CYP6AA2 from Anopheles minimus resistant to deltamethrin
Issued Date
2003-01-01
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ISSN
10811710
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2-s2.0-2142767038
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Vector Ecology. Vol.28, No.2 (2003), 150-158
Suggested Citation
Pornpimol Rongnoparut, Soamrutai Boonsuepsakul, Theeraphap Chareonviriyaphap, Naowarat Thanomsing Cloning of cytochrome P450, CYP6P5, and CYP6AA2 from Anopheles minimus resistant to deltamethrin. Journal of Vector Ecology. Vol.28, No.2 (2003), 150-158. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20659
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Title
Cloning of cytochrome P450, CYP6P5, and CYP6AA2 from Anopheles minimus resistant to deltamethrin
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Abstract
Two new genes in the cytochrome P450 (CYP6) family 6 with complete coding sequences were cloned and sequenced from deltamethrin-resistant Anopheles minimus, a major malaria vector in Thailand. CYP6P5 encodes a protein of 508 amino acids, while CYP6AA2 contains 505 residues. Each encoded protein contains a hydrophobic N-terminal region and a highly conserved herne-binding region typical of P450s. Alignments of deduced amino acid sequences with other insect P450 genes indicate a high degree of identity to insect CYP6 genes. Comparative mRNA expression studies using semi-quantitative RT-PCR analysis indicated that the relative amount of CYP6AA2 transcript was greater in the deltamethrin-resistant An. minimus compared to the susceptible strain. The expression of CYP6AA2 in deltamethrin-resistant mosquitoes is associated with development of deltamethrin resistance in An. minimus mosquito. The CYP6P5 transcript is equally expressed in both resistant and susceptible mosquitoes.