Publication: Monocyte activation by Porphyromonas gingivalis LPS in aggressive periodontitis with the use of whole-blood cultures
Issued Date
2004-01-01
Resource Type
ISSN
00220345
Other identifier(s)
2-s2.0-4143098465
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Dental Research. Vol.83, No.7 (2004), 540-545
Suggested Citation
R. Mahanonda, N. Sa-Ard-Iam, O. Charatkulangkun, A. Promsudthi, R. E. Schifferle, K. Yongvanichit, S. Pichyangkul Monocyte activation by Porphyromonas gingivalis LPS in aggressive periodontitis with the use of whole-blood cultures. Journal of Dental Research. Vol.83, No.7 (2004), 540-545. doi:10.1177/154405910408300706 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21304
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Title
Monocyte activation by Porphyromonas gingivalis LPS in aggressive periodontitis with the use of whole-blood cultures
Abstract
In this study, we re-visited the issue of hyper-responsiveness of monocytes to bacterial lipopolysaccharide (LPS) in aggressive periodontitis patients. We used whole-blood cultures to compare monocyte activation by Porphyromonas gingivalis LPS between Thai subjects with generalized aggressive periodontitis and those without periodontitis. Upon stimulation with P. gingivalis LPS, expression of co-stimulatory molecules on monocytes and expression of CD69 on NK and γδ T-cells were analyzed by flow cytometry, and the production of interleukin-1β and prostaglandin E2was monitored by ELISA. LPS stimulation resulted in a dose-dependent up-regulation of CD40, CD80, and CD86 on monocytes, and up-regulation of CD69 on NK cells and γδ T-cells in both the periodontitis and non-periodontitis groups. The levels of activation markers and the mediator production after LPS stimulation were quite similar for both groups. In conclusion, we did not observe hyper-responsiveness of monocytes to P. gingivalis LPS challenge in Thai patients with aggressive periodontitis.