Publication: Leptospirosis research: Fast, easy and reliable enumeration of mobile leptospires
Issued Date
2009-01-01
Resource Type
ISSN
07176287
07169760
07169760
Other identifier(s)
2-s2.0-70350007203
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Mahidol University
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SCOPUS
Bibliographic Citation
Biological Research. Vol.42, No.1 (2009), 5-12
Suggested Citation
Stefan Schreier, Wannapong Triampo, Galayanee Doungchawee, Darapond Triampo, Sudarat Chadsuthi Leptospirosis research: Fast, easy and reliable enumeration of mobile leptospires. Biological Research. Vol.42, No.1 (2009), 5-12. doi:10.4067/S0716-97602009000100001 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27072
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Title
Leptospirosis research: Fast, easy and reliable enumeration of mobile leptospires
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Abstract
Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for the establishment of standard curves. They were found to be linear and slightly different in gradient for each serovar. The ease, rapidity, and reliability of these two adapted and optimized counting techniques may provide a useful alternative enumeration technique for leptospirosis research.