Production, purification and characterization of thermophilic lipase from Bacillus sp. THL027

Abstract

A low-cost medium, MGRS, has been developed for growth and lipase production from Bacillus THL027 at 65°C and pH 7.0. MGRS was composed of 2% (v/v) buffer solution (7.3% (w/v) Na2HPO4, 3.2% (w/v) KH2PO4, pH 7.2), 40 μg ml-1FeSO4and 40 μg ml-1MgSO4, 0.1% (w/v) (NH4)2SO4supplemented with 3% NaCl, 0.1% glucose, 1.0% rice bran oil and 0.5% (w/v) rice bran. The lipase was purified 2.6-fold to apparent homogeneity by ultrafiltration and gel filtration chromatography. Its molecular mass was 69 kDa. The purified enzyme was characterized for its general physical properties. Copyright (C) 1999 Federation of European Microbiological Societies.