Publication: Phylogenetic analysis of Ara<sup>+</sup> and Ara<sup>-</sup> Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes
Issued Date
1999-05-29
Resource Type
ISSN
00951137
Other identifier(s)
2-s2.0-0032929879
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Clinical Microbiology. Vol.37, No.6 (1999), 1906-1912
Suggested Citation
Tararaj Dharakul, Boonratn Tassaneetrithep, Suwanna Trakulsomboon, Sirirurg Songsivilai Phylogenetic analysis of Ara<sup>+</sup> and Ara<sup>-</sup> Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes. Journal of Clinical Microbiology. Vol.37, No.6 (1999), 1906-1912. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/25444
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Phylogenetic analysis of Ara<sup>+</sup> and Ara<sup>-</sup> Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes
Other Contributor(s)
Abstract
A Burkholderia pseudomallei-like organism has recently been identified among some soil isolates of B. pseudomallei in an area with endemic melioidosis. This organism is almost identical to B. pseudomallei in terms of morphological and biochemical profiles, except that it differs in ability to assimilate L-arabinose. These Ara+ isolates are also less virulent than the Ara- isolates in animal models. In addition, clinical isolates of B. pseudomallei available to date are almost exclusively Ara-. These features suggested that these two organisms may belong to distinctive species. In this study, the 16S rRNA-encoding genes from five clinical (four Ara- and one Ara+) and nine soil isolates (five Ara- and four Ara+) of B. pseudomallei were sequenced. The nucleotide sequences and phylogenetic analysis indicated that the 16S rRNA-encoding gene of the Ara+ biotype was similar to but distinctively different from that of the Ara- soil isolates, which were identical to the classical clinical isolates of B. pseudomallei. The nucleotide sequence differences in the 16S rRNA-encoding gene appeared to be specific for the Ara+ or Ara- biotypes. The differences were, however, not sufficient for classification into a new species within the genus Burkholderia. A simple and rapid multiplex PCR procedure was developed to discriminate between Ara- and Ara+ B. pseudomallei isolates. This new method could also be incorporated into our previously reported nested PCR system for detecting B. pseudomallei in clinical specimens.