Publication:
Induction of Meristematic Nodular Calli from Various Explants of Lilium spp. and Long Term Stability in Plant Regeneration Ability and Ploidy Level of the Calli

1

Issued Date

1998-01-01

Resource Type

Article

ISSN

13476114
13424580

DOI

10.5511/plantbiotechnology.15.95

Other identifier(s)

2-s2.0-54649083077

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Plant Biotechnology. Vol.15, No.2 (1998), 95-102

Suggested Citation

Kanyaratt Supaibulwatana, Masahiro Mii Induction of Meristematic Nodular Calli from Various Explants of Lilium spp. and Long Term Stability in Plant Regeneration Ability and Ploidy Level of the Calli. Plant Biotechnology. Vol.15, No.2 (1998), 95-102. doi:10.5511/plantbiotechnology.15.95 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18265

Research Projects

Organizational Units

Authors

Journal Issue

Thesis

Title

Induction of Meristematic Nodular Calli from Various Explants of Lilium spp. and Long Term Stability in Plant Regeneration Ability and Ploidy Level of the Calli

Author(s)

Kanyaratt Supaibulwatana
 Masahiro Mii

Other Contributor(s)

Chiba University
 Mahidol University

Abstract

Meristematic nodular calli (NOD) were successfully induced from various plant parts such as seeds, flower organs, shoot apices, stem segments and bulb scales of Lilium spp. on media containing 1 mg/l picloram alone or in combination with 1 mg/l cytokinin. In L. longiflorum, green friable callus (FC) was also induced from bulb scales. Suspension cultures established from both NOD and FC showed high proliferation rate. NOD retained high regeneration ability as well as stable ploidy level during the culture for more than 4 years, while the suspension culture of FC became tetraploid. Protoplasts were efficiently isolated from NOD in some species and cultivars after long term subculture in suspension cultures and the plants were successfully regenerated from the protoplasts of L. x formolongi.

Keyword(s)

Agricultural and Biological Sciences
 Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/18265

Collections

Scopus 1991-2000