Publication: Roles of Agrobacterium tumefaciens C58 ZntA and ZntB and the transcriptional regulator ZntR in controlling Cd<sup>2+</sup>/Zn<sup>2+</sup>/Co<sup>2+</sup> resistance and the peroxide stress response
Issued Date
2015-01-01
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ISSN
14652080
13500872
13500872
Other identifier(s)
2-s2.0-84943653000
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Mahidol University
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SCOPUS
Bibliographic Citation
Microbiology (United Kingdom). Vol.161, No.9 (2015), 1730-1740
Suggested Citation
Paweena Chaoprasid, Sumontha Nookabkaew, Rojana Sukchawalit, Skorn Mongkolsuk Roles of Agrobacterium tumefaciens C58 ZntA and ZntB and the transcriptional regulator ZntR in controlling Cd<sup>2+</sup>/Zn<sup>2+</sup>/Co<sup>2+</sup> resistance and the peroxide stress response. Microbiology (United Kingdom). Vol.161, No.9 (2015), 1730-1740. doi:10.1099/mic.0.000135 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/36132
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Title
Roles of Agrobacterium tumefaciens C58 ZntA and ZntB and the transcriptional regulator ZntR in controlling Cd<sup>2+</sup>/Zn<sup>2+</sup>/Co<sup>2+</sup> resistance and the peroxide stress response
Abstract
© 2015 The Authors. The putative zinc exporters ZntA (a P1B-type ATPase) and ZntB (2-TM-GxN family) in Agrobacterium tumefaciens were characterized. The expression of the zntA gene is inducible by CdCl2, ZnCl2and CoCl2, of which CdCl2is the most potent inducer, whereas zntB is constitutively expressed. The metal-induced expression of zntA is controlled by the MerR-like regulator ZntR. The zntA and zntR mutants were highly sensitive to CdCl2and ZnCl2, and CoCl2sensitivity was demonstrated to a lesser extent. By contrast, the zntB mutant showed similar levels of metal resistance to the WT strain. Even in the zntA mutant background, zntB did not play an apparent role in metal resistance under the conditions tested. The inactivation of zntA increased the accumulation of intracellular cadmium and zinc, and conferred hyperresistance to H2O2. Thus, the metal transporter ZntA and its regulator ZntR are important for controlling zinc homeostasis and cadmium and cobalt detoxification. The loss of either the zntA or zntR gene did not affect the virulence of A. tumefaciens in Nicotiana benthamiana.