Publication: A simple and rapid microplate fluorescence determination of adamantanes in pharmaceutical formulations
Issued Date
2019-01-01
Resource Type
ISSN
13369075
03666352
03666352
Other identifier(s)
2-s2.0-85076918294
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Chemical Papers. (2019)
Suggested Citation
Nantana Nuchtavorn, Chatchanon Sudprasert, Peeradon Yurai, Leena Suntornsuk A simple and rapid microplate fluorescence determination of adamantanes in pharmaceutical formulations. Chemical Papers. (2019). doi:10.1007/s11696-019-01035-x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50310
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
A simple and rapid microplate fluorescence determination of adamantanes in pharmaceutical formulations
Other Contributor(s)
Abstract
© 2019, Institute of Chemistry, Slovak Academy of Sciences. Abstract: Adamantane drugs (e.g., memantine, rimantadine, and amantadine) consist of a core tricyclodecane with different substituents. Memantine is used for treatment of Alzheimer’s disease, while rimantadine and amantadine are recommended for influenza A infection. Additionally, amantadine is clinically used for Parkinson’s disease. Analysis of adamantine drugs by optical detection requires some derivatization due to the lack of chromophores or fluorophores in their structures. This procedure is performed to enhance their detectability, but it can be time- and labor-intensive. This work focuses on developments of a simple and fast technique for the analysis of these drugs in a microplate platform. Derivatization was achieved in 50 mM borate buffer (pH 11.0) using a stoichiometric ratio between the analyte and fluorescamine (as a derivatizing reagent) of 1:10 and a reaction time of 10 min. The fluorescent derivatives were determined in a 96-well microplate using excitation and emission wavelengths at 385 and 485 nm, respectively. The method showed good linearity (r2 > 0.968), repeatability (RSDs of < 2.6%), and accuracy (% recovery 96.2–101.1 with RSD < 4.5%) with acceptable limits of detection (< 3 µM) and quantitation (< 10 µM). The method was successfully applied for the determination of the drugs in pharmaceutical formulations. Graphic abstract: [Figure not available: see fulltext.].