Publication: Cytotoxin and hemolysin in diseased fish during epidemic outbreaks
Issued Date
1987-01-01
Resource Type
ISSN
10982256
08848181
08848181
Other identifier(s)
2-s2.0-0023182297
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Mahidol University
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SCOPUS
Bibliographic Citation
Toxicity Assessment. Vol.2, No.1 (1987), 97-103
Suggested Citation
Laddavan Laohaviranit Cytotoxin and hemolysin in diseased fish during epidemic outbreaks. Toxicity Assessment. Vol.2, No.1 (1987), 97-103. doi:10.1002/tox.2540020109 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15474
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Title
Cytotoxin and hemolysin in diseased fish during epidemic outbreaks
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Abstract
Outbreaks of fish diseases have been reported in many parts of the world. The outbreaks are difficult to control. During December 1982 and March 1983, there were severe outbreaks of fish disease in Thailand. A study on the presence and inactivation of harmful toxins, i.e., cytotoxin and hemolysin, by heat, salt and gastric pH was undertaken. Cytotoxin and hemolysin were detected in all diseased snakehead fish (Ophicephalus striatus) homogenates. Aeromonas hydrophila F 588 isolated from the diseased snakehead fish also produced cytotoxin and hemolysin. No detectable cytotoxic or hemolytic activity was found in the fish homogenates or A. hydrophila F 588 cell suspensions after heating at 100°C for 5 minutes or autoclaving at 121°C for 15 minutes at 15 lb/in. 2 Cytotoxic activity remained positive in all concentrations (0% to 30% W/V) of NaCl. However, no cytotoxin could be detected when the pH of the samples was 2.0. There was a 55% decrease in hemolytic activity when A. hydrophila F 588 was incubated in 30% NaCl for 1 month at 30°C. Furthermore, there was a 50% decrease in the activity when the pH of the samples was 2.0. Hence, the diseased fish is safe for consumption if it is heated for 5 minutes at 100°C. However, it is unsafe to consume unheated salt‐fermented fish. Copyright © 1987 Wiley Subscription Services, Inc., A Wiley Company