Publication: Identification of New Protein Interactions between Dengue Fever Virus and Its Hosts, Human and Mosquito
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Issued Date
2013-01-17
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ISSN
19326203
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2-s2.0-84872245786
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS ONE. Vol.8, No.1 (2013)
Suggested Citation
Dumrong Mairiang, Huamei Zhang, Ann Sodja, Thilakam Murali, Prapat Suriyaphol, Prida Malasit, Thawornchai Limjindaporn, Russell L. Finley Identification of New Protein Interactions between Dengue Fever Virus and Its Hosts, Human and Mosquito. PLoS ONE. Vol.8, No.1 (2013). doi:10.1371/journal.pone.0053535 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31085
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Title
Identification of New Protein Interactions between Dengue Fever Virus and Its Hosts, Human and Mosquito
Abstract
The four divergent serotypes of dengue virus are the causative agents of dengue fever, dengue hemorrhagic fever and dengue shock syndrome. About two-fifths of the world's population live in areas where dengue is prevalent, and thousands of deaths are caused by the viruses every year. Dengue virus is transmitted from one person to another primarily by the yellow fever mosquito, Aedes aegypti. Recent studies have begun to define how the dengue viral proteins interact with host proteins to mediate viral replication and pathogenesis. A combined analysis of these studies, however, suggests that many virus-host protein interactions remain to be identified, especially for the mosquito host. In this study, we used high-throughput yeast two-hybrid screening to identify mosquito and human proteins that physically interact with dengue proteins. We tested each identified host protein against the proteins from all four serotypes of dengue to identify interactions that are conserved across serotypes. We further confirmed many of the interactions using co-affinity purification assays. As in other large-scale screens, we identified some previously detected interactions and many new ones, moving us closer to a complete host - dengue protein interactome. To help summarize and prioritize the data for further study, we combined our interactions with other published data and identified a subset of the host-dengue interactions that are now supported by multiple forms of evidence. These data should be useful for understanding the interplay between dengue and its hosts and may provide candidates for drug targets and vector control strategies. © 2013 Mairiang et al.