Publication: Human embryonic stem cells derived by somatic cell nuclear transfer
2
Issued Date
2013-06-06
Resource Type
ISSN
10974172
00928674
00928674
Other identifier(s)
2-s2.0-84878838747
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Cell. Vol.153, No.6 (2013), 1228-1238
Suggested Citation
Masahito Tachibana, Paula Amato, Michelle Sparman, Nuria Marti Gutierrez, Rebecca Tippner-Hedges, Hong Ma, Eunju Kang, Alimujiang Fulati, Hyo Sang Lee, Hathaitip Sritanaudomchai, Keith Masterson, Janine Larson, Deborah Eaton, Karen Sadler-Fredd, David Battaglia, David Lee, Diana Wu, Jeffrey Jensen, Phillip Patton, Sumita Gokhale, Richard L. Stouffer, Don Wolf, Shoukhrat Mitalipov Human embryonic stem cells derived by somatic cell nuclear transfer. Cell. Vol.153, No.6 (2013), 1228-1238. doi:10.1016/j.cell.2013.05.006 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31295
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Title
Human embryonic stem cells derived by somatic cell nuclear transfer
Author(s)
Masahito Tachibana
Paula Amato
Michelle Sparman
Nuria Marti Gutierrez
Rebecca Tippner-Hedges
Hong Ma
Eunju Kang
Alimujiang Fulati
Hyo Sang Lee
Hathaitip Sritanaudomchai
Keith Masterson
Janine Larson
Deborah Eaton
Karen Sadler-Fredd
David Battaglia
David Lee
Diana Wu
Jeffrey Jensen
Phillip Patton
Sumita Gokhale
Richard L. Stouffer
Don Wolf
Shoukhrat Mitalipov
Paula Amato
Michelle Sparman
Nuria Marti Gutierrez
Rebecca Tippner-Hedges
Hong Ma
Eunju Kang
Alimujiang Fulati
Hyo Sang Lee
Hathaitip Sritanaudomchai
Keith Masterson
Janine Larson
Deborah Eaton
Karen Sadler-Fredd
David Battaglia
David Lee
Diana Wu
Jeffrey Jensen
Phillip Patton
Sumita Gokhale
Richard L. Stouffer
Don Wolf
Shoukhrat Mitalipov
Abstract
Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limitations allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT-ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state. PaperClip © 2013 Elsevier Inc.