Publication:
A molecular marker of artemisinin-resistant Plasmodium falciparum malaria

1

Issued Date

2014-01-01

Resource Type

Article

ISSN

14764687
00280836

DOI

10.1038/nature12876

Other identifier(s)

2-s2.0-84892372929

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Nature. Vol.505, No.7481 (2014), 50-55

Suggested Citation

Frédéric Ariey, Benoit Witkowski, Chanaki Amaratunga, Johann Beghain, Anne Claire Langlois, Nimol Khim, Saorin Kim, Valentine Duru, Christiane Bouchier, Laurence Ma, Pharath Lim, Rithea Leang, Socheat Duong, Sokunthea Sreng, Seila Suon, Char Meng Chuor, Denis Mey Bout, Sandie Ménard, William O. Rogers, Blaise Genton, Thierry Fandeur, Olivo Miotto, Pascal Ringwald, Jacques Le Bras, Antoine Berry, Jean Christophe Barale, Rick M. Fairhurst, Françoise Benoit-Vical, Odile Mercereau-Puijalon, Didier Ménard A molecular marker of artemisinin-resistant Plasmodium falciparum malaria. Nature. Vol.505, No.7481 (2014), 50-55. doi:10.1038/nature12876 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/34983

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Title

A molecular marker of artemisinin-resistant Plasmodium falciparum malaria

Author(s)

Frédéric Ariey
 Benoit Witkowski
 Chanaki Amaratunga
 Johann Beghain
 Anne Claire Langlois
 Nimol Khim
 Saorin Kim
 Valentine Duru
 Christiane Bouchier
 Laurence Ma
 Pharath Lim
 Rithea Leang
 Socheat Duong
 Sokunthea Sreng
 Seila Suon
 Char Meng Chuor
 Denis Mey Bout
 Sandie Ménard
 William O. Rogers
 Blaise Genton
 Thierry Fandeur
 Olivo Miotto
 Pascal Ringwald
 Jacques Le Bras
 Antoine Berry
 Jean Christophe Barale
 Rick M. Fairhurst
 Françoise Benoit-Vical
 Odile Mercereau-Puijalon
 Didier Ménard

Other Contributor(s)

Institut Pasteur, Paris
 CNRS Centre National de la Recherche Scientifique
 Institut Pasteur du Cambodge
 National Institute of Allergy and Infectious Diseases
 National Center for Parasitology, Entomology and Malaria Control
 CHU de Toulouse
 Naval Medical Research Unit-2
 Swiss Tropical and Public Health Institute (Swiss TPH)
 University of Oxford
 Mahidol University
 Wellcome Trust Sanger Institute
 Organisation Mondiale de la Sante
 Hopital Bichat-Claude-Bernard AP-HP
 Universite de Toulouse

Abstract

Plasmodium falciparum resistance to artemisinin derivatives in southeast Asia threatens malaria control and elimination activities worldwide. To monitor the spread of artemisinin resistance, a molecular marker is urgently needed. Here, using whole-genome sequencing of an artemisinin-resistant parasite line from Africa and clinical parasite isolates from Cambodia, we associate mutations in the PF3D7-1343700 kelch propeller domain ('K13-propeller') with artemisinin resistance in vitro and in vivo. Mutant K13-propeller alleles cluster in Cambodian provinces where resistance is prevalent, and the increasing frequency of a dominant mutant K13-propeller allele correlates with the recent spread of resistance in western Cambodia. Strong correlations between the presence of a mutant allele, in vitro parasite survival rates and in vivo parasite clearance rates indicate that K13-propeller mutations are important determinants of artemisinin resistance. K13-propeller polymorphism constitutes a useful molecular marker for large-scale surveillance efforts to contain artemisinin resistance in the Greater Mekong Subregion and prevent its global spread. © 2014 Macmillan Publishers Limited.

Keyword(s)

Multidisciplinary

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URI

https://repository.li.mahidol.ac.th/handle/123456789/34983

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