Publication: A molecular marker of artemisinin-resistant Plasmodium falciparum malaria
Issued Date
2014-01-01
Resource Type
ISSN
14764687
00280836
00280836
Other identifier(s)
2-s2.0-84892372929
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Mahidol University
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SCOPUS
Bibliographic Citation
Nature. Vol.505, No.7481 (2014), 50-55
Suggested Citation
Frédéric Ariey, Benoit Witkowski, Chanaki Amaratunga, Johann Beghain, Anne Claire Langlois, Nimol Khim, Saorin Kim, Valentine Duru, Christiane Bouchier, Laurence Ma, Pharath Lim, Rithea Leang, Socheat Duong, Sokunthea Sreng, Seila Suon, Char Meng Chuor, Denis Mey Bout, Sandie Ménard, William O. Rogers, Blaise Genton, Thierry Fandeur, Olivo Miotto, Pascal Ringwald, Jacques Le Bras, Antoine Berry, Jean Christophe Barale, Rick M. Fairhurst, Françoise Benoit-Vical, Odile Mercereau-Puijalon, Didier Ménard A molecular marker of artemisinin-resistant Plasmodium falciparum malaria. Nature. Vol.505, No.7481 (2014), 50-55. doi:10.1038/nature12876 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/34983
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Title
A molecular marker of artemisinin-resistant Plasmodium falciparum malaria
Author(s)
Frédéric Ariey
Benoit Witkowski
Chanaki Amaratunga
Johann Beghain
Anne Claire Langlois
Nimol Khim
Saorin Kim
Valentine Duru
Christiane Bouchier
Laurence Ma
Pharath Lim
Rithea Leang
Socheat Duong
Sokunthea Sreng
Seila Suon
Char Meng Chuor
Denis Mey Bout
Sandie Ménard
William O. Rogers
Blaise Genton
Thierry Fandeur
Olivo Miotto
Pascal Ringwald
Jacques Le Bras
Antoine Berry
Jean Christophe Barale
Rick M. Fairhurst
Françoise Benoit-Vical
Odile Mercereau-Puijalon
Didier Ménard
Benoit Witkowski
Chanaki Amaratunga
Johann Beghain
Anne Claire Langlois
Nimol Khim
Saorin Kim
Valentine Duru
Christiane Bouchier
Laurence Ma
Pharath Lim
Rithea Leang
Socheat Duong
Sokunthea Sreng
Seila Suon
Char Meng Chuor
Denis Mey Bout
Sandie Ménard
William O. Rogers
Blaise Genton
Thierry Fandeur
Olivo Miotto
Pascal Ringwald
Jacques Le Bras
Antoine Berry
Jean Christophe Barale
Rick M. Fairhurst
Françoise Benoit-Vical
Odile Mercereau-Puijalon
Didier Ménard
Other Contributor(s)
Institut Pasteur, Paris
CNRS Centre National de la Recherche Scientifique
Institut Pasteur du Cambodge
National Institute of Allergy and Infectious Diseases
National Center for Parasitology, Entomology and Malaria Control
CHU de Toulouse
Naval Medical Research Unit-2
Swiss Tropical and Public Health Institute (Swiss TPH)
University of Oxford
Mahidol University
Wellcome Trust Sanger Institute
Organisation Mondiale de la Sante
Hopital Bichat-Claude-Bernard AP-HP
Universite de Toulouse
CNRS Centre National de la Recherche Scientifique
Institut Pasteur du Cambodge
National Institute of Allergy and Infectious Diseases
National Center for Parasitology, Entomology and Malaria Control
CHU de Toulouse
Naval Medical Research Unit-2
Swiss Tropical and Public Health Institute (Swiss TPH)
University of Oxford
Mahidol University
Wellcome Trust Sanger Institute
Organisation Mondiale de la Sante
Hopital Bichat-Claude-Bernard AP-HP
Universite de Toulouse
Abstract
Plasmodium falciparum resistance to artemisinin derivatives in southeast Asia threatens malaria control and elimination activities worldwide. To monitor the spread of artemisinin resistance, a molecular marker is urgently needed. Here, using whole-genome sequencing of an artemisinin-resistant parasite line from Africa and clinical parasite isolates from Cambodia, we associate mutations in the PF3D7-1343700 kelch propeller domain ('K13-propeller') with artemisinin resistance in vitro and in vivo. Mutant K13-propeller alleles cluster in Cambodian provinces where resistance is prevalent, and the increasing frequency of a dominant mutant K13-propeller allele correlates with the recent spread of resistance in western Cambodia. Strong correlations between the presence of a mutant allele, in vitro parasite survival rates and in vivo parasite clearance rates indicate that K13-propeller mutations are important determinants of artemisinin resistance. K13-propeller polymorphism constitutes a useful molecular marker for large-scale surveillance efforts to contain artemisinin resistance in the Greater Mekong Subregion and prevent its global spread. © 2014 Macmillan Publishers Limited.