Publication: Phytoestrogens mediated anti-inflammatory effect through suppression of IRF-1 and pSTAT1 expressions in lipopolysaccharide-activated microglia
Issued Date
2013-01-01
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ISSN
18781705
15675769
15675769
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2-s2.0-84885359067
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Mahidol University
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SCOPUS
Bibliographic Citation
International Immunopharmacology. Vol.17, No.2 (2013), 483-488
Suggested Citation
Nattinee Jantaratnotai, Pongsak Utaisincharoen, Pimtip Sanvarinda, Anusorn Thampithak, Yupin Sanvarinda Phytoestrogens mediated anti-inflammatory effect through suppression of IRF-1 and pSTAT1 expressions in lipopolysaccharide-activated microglia. International Immunopharmacology. Vol.17, No.2 (2013), 483-488. doi:10.1016/j.intimp.2013.07.013 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31983
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Title
Phytoestrogens mediated anti-inflammatory effect through suppression of IRF-1 and pSTAT1 expressions in lipopolysaccharide-activated microglia
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Abstract
Microglial activation has been implicated in various neurological disorders, including Alzheimer's disease, Parkinson's disease, multiple sclerosis, and HIV encephalopathy. Phytoestrogens have been shown to be neuroprotective in neurotoxicity models; however, their effect on microglia has not been well established. In the current study, we report that the soy phytoestrogens, genistein, daidzein, and coumestrol, decreased nitric oxide (NO) production induced by lipopolysaccharide (LPS) in the rat microglial cell line (HAPI). The levels of inducible NO synthase (iNOS) mRNA and protein expression were also reduced. Transcription factors known to govern iNOS expression including interferon regulatory factor-1 (IRF-1) and phosphorylated STAT1 were down regulated. These observations explain, at least in part, the inhibitory effect of phytoestrogens on NO production. The levels of monocyte chemoattractant protein-1 and interleukin-6 mRNA, proinflammatory chemokine and cytokine associated with various neurological disorders, were also reduced following LPS stimulation when HAPI cells were pretreated with phytoestrogens. Hence, genistein, daidzein, and coumestrol could serve as anti-inflammatory agents and may have beneficial effects in the treatment of neurodegenerative diseases. © 2013 Elsevier B.V. All rights reserved.